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目的通过检测河南省0～12岁健康儿童、手足口病痊愈病人、手足口病现症病人血清肠道病毒71型(EV71)抗体水平,了解手足口病优势病毒株EV71人群感染情况,为预防控制手足口病等提供科学依据。方法在河南省不同地区采集0～12岁健康儿童、手足口病痊愈病人和手足口病现症病人血清共226份,采用微量中和滴定法检测抗EV71抗体。结果健康儿童、手足口病痊愈病人和手足口病现症病人血清EV71中和抗体阳性率分别为51.46%、92.96%和55.77%,差异有统计学意义(P<0.05),EV71几何平均滴度(GMT)分别为11.13(8.83～13.99)、66.51(52.09～84.97)和21.93(14.32～33.57),差异有统计学意义(P<0.05)。结论手足口病流行地区健康儿童血清EV71中和抗体阳性率较高,EV71阴性感染较普遍。手足口病康复病人能够产生较高滴度的免疫保护性抗体,对EV71病毒再次感染具有一定的保护作用。
Objective To detect the level of antibody against EV71 in healthy children aged 0-12 years and recovered from hand-foot-mouth disease in Henan Province, and to know the prevalence of EV71 in patients with hand-foot-mouth disease Control hand, foot and mouth disease provide scientific basis. Methods A total of 226 serum samples from healthy children aged 0-12 years, patients with HFMD and patients with HFMD were collected from different areas in Henan Province. Anti-EV71 antibody was detected by micro-neutralization titration. Results The positive rates of serum neutralizing antibodies against EV71 in healthy children, patients with HFMD and those with HFMD were 51.46%, 92.96% and 55.77%, respectively, with significant difference (P <0.05). The geometric mean titer of EV71 (GMT) were 11.13 (8.83-13.99), 66.51 (52.09-84.97) and 21.93 (14.32-33.57), respectively. The difference was statistically significant (P <0.05). Conclusion The positive rate of serum EV71 neutralizing antibody in healthy children with HFMD is high, and the EV71 negative infection is more common. Hand, foot and mouth disease patients can produce a higher titer of immune protective antibodies, re-infection of EV71 virus has a protective effect.