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目的探讨ＤＮＡ载体结构及接种途径对ＤＮＡ疫苗免疫效果的影响。方法分别构建插入ＨＢＶ表面抗原编码基因的表达载体ｐｃＤＮＡ１．１／ＳＡ（无抗性基因）和ｐｃＤＮＡＩ／Ａｍｐ／ＳＡ（含氨苄青霉素抗性基因），肌注小鼠后比较其诱生特异性免疫应答的能力；同时比较不同接种途径（肌内、皮内、皮肤划痕）及ＣｐＧ免疫刺激元件（ＩＳＳ）对ＤＮＡ疫苗诱生免疫效果的影响。结果ｐｃＤＮＡＩ／Ａｍｐ／ＳＡ的免疫效果优于ｐｃＤＮＡ１．１／ＳＡ。ｐｃＤＮＡ１．１／ＳＡ的免疫效果可被ＩＳＳ增强，而ｐｃＤＮＡＩ／Ａｍｐ／ＳＡ诱生特异性免疫应答的能力则可被ＩＳＳ抑制；诱生免疫应答的能力以肌内注射最强，皮内注射免疫其次，皮下划痕法较弱。结论不同ＨＢｓＡｇ表达载体诱生免疫应答的能力不尽相同；ＣｐＧ免疫刺激元件在决定ＤＮＡ疫苗免疫原性中起重要作用，可增强不含相应结构ＤＮＡ疫苗的免疫效果；皮内注射可诱发与肌内接种相似的免疫应答，是一种简便、有效的免疫接种途径
Objective To investigate the effect of DNA vector structure and immunization pathways on DNA vaccine immunization. Methods The recombinant plasmid pcDNA1.1 / SA (pcDNA1.1 / SA) and pcDNAI / Amp / SA (ampicillin resistant gene) were constructed and inserted into the surface antigen-specific genes Response ability of different vaccination routes (intramuscular, intradermal, skin scratches) and CpG immune stimulating elements (ISS) on the immune effect induced by DNA vaccine. Results The immunogenicity of pcDNAI / Amp / SA was better than that of pcDNA1.1 / SA. The immune effect of pcDNA1.1 / SA can be enhanced by ISS, while the ability of pcDNAI / Amp / SA to induce specific immune responses can be inhibited by ISS; the ability to induce an immune response is highest in intramuscular injection and intradermally injections Second, the subcutaneous scarring method is weak. Conclusion Different HBsAg expression vectors have different abilities to induce immune responses. CpG immunostimulatory elements play an important role in determining the immunogenicity of DNA vaccines, and can enhance the immunogenicity of DNA vaccines without corresponding DNA vaccines. Inoculation of similar immune responses is a simple and effective route of immunization