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目的探讨肝脂酶(HL)基因启动子区-514C/T多态性与糖尿病慢性肾脏疾病(CKD)的相关性。方法纳入T2DM患者(T2DM组)164例,根据UAlb水平再分为CKD亚组(CKD,n=108)和无CKD亚组(CKD0,n=56);另选健康对照者(NC组)91名。运用限制性片段长度多态性(PCR-RFLP)技术对各组HL基因启动子区-514C/T多态性进行检测,并比较各组间基因型和等位基因频率及相关资料。结果 (1)CKD亚组C/T+T/T基因型和T等位基因频率高于CKD0亚组(P<0.05);(2)NC组与T2DM组间基因型和等位基因频率差异无统计学意义(P>0.05);(3)T2DM组中C/T+T/T基因型患者TG水平高于C/C基因型患者(P<0.05);(4)Logistic回归分析表明,HL基因启动子区-514C/T基因多态性与CKD相关。结论 HL基因启动子区-514T等位基因可能是CKD发生的遗传易感因素。
Objective To investigate the association between -514C / T polymorphism of hepatic lipase (HL) gene promoter region and chronic kidney disease (CKD) in diabetes mellitus. Methods One hundred and sixty-four patients with T2DM were enrolled in this study. According to the level of UAlb, they were subdivided into CKD subgroup (CKD, n = 108) and non-CKD subgroup (CKD0, n = 56) name. The -514C / T polymorphism of promoter region of HL gene in each group was detected by restriction fragment length polymorphism (PCR-RFLP), and the genotype and allele frequency and related data were compared between the groups. Results (1) The frequencies of C / T + T / T genotype and T allele in CKD subgroup were higher than that in CKD subgroup (P <0.05). (2) The frequencies of genotype and allele in NC group and T2DM group were significantly different (3) The TG level of C / T + T / T genotype in T2DM group was higher than that of C / C genotype (P <0.05); (4) Logistic regression analysis showed that the level of TG in C / The -514C / T polymorphism of HL gene promoter region is associated with CKD. Conclusion -514T allele of HL gene promoter may be a predisposing factor for CKD.