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目的研究肝癌转铁蛋白受体 (TfR)的基因表达及其单克隆抗体免疫诊断肝癌的价值。 方法对 2 0例肝癌手术标本包括癌巢组织、癌旁组织及周围正常组织进行Northernblot分析 ,了解三种组织TfRmRNA表达情况 ;通过间接免疫荧光及免疫组织化学ABC法了解肝癌细胞和正常肝细胞膜上TfR表达情况。 结果TfRmRNA表达癌组织最高 ,癌旁组织次之 ,周围正常组织最低 ,后两者间无显著差异 ;肝癌细胞膜转铁蛋白受体荧光阳性率明显高于正常肝细胞 ,两者有显著差异。免疫组织化学ABC法中 1 9例强阳性染色 ,1例阴性。 结论转铁蛋白受体的基因表达在肝癌组织中明显增高 ,其单克隆抗体有希望成为肝癌免疫诊断的理想载体
Objective To study the gene expression of liver transferrin receptor (TfR) and the value of its monoclonal antibody in the diagnosis of liver cancer. Methods Northern blot analysis was performed on 20 liver cancer surgical specimens, including cancer nest tissues, paracancerous tissues, and surrounding normal tissues to understand the expression of TfR mRNA in three tissues. The indirect immunofluorescence and immunohistochemical ABC methods were used to understand the hepatoma cells and normal liver cell membranes. TfR expression. Results The expression of TfR mRNA was highest in cancer tissues, followed by adjacent tissues, and lowest in surrounding normal tissues. There was no significant difference between the latter two groups. The positive rate of liver cell membrane transferrin receptor fluorescence was significantly higher than that of normal liver cells. There was a significant difference between them. Immunohistochemistry ABC method in 19 cases of strong positive staining, 1 case was negative. Conclusion The gene expression of transferrin receptor is significantly increased in hepatocellular carcinoma, and its monoclonal antibody is expected to be an ideal carrier for immunodiagnosis of liver cancer.