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目的: 研究中西医结合治疗后, SARS患者康复期淋巴细胞功能状态和T细胞受体(TCR)Vβ24个亚家族表达的格局。方法: 应用流式细胞分析技术, 观察我院 76例康复期患者T细胞亚群, T、B细胞的活化状态及CD3+和TCRVβ1、2、3、4、5. 1、5. 2、5. 3、7. 1、7. 2、8、9、11、12、13. 1、13. 2、13. 6、14、16、17、18、20、21. 3、22及 23等 24个亚家族的表达。结果: T细胞亚群中, CD3+和CD4+ T细胞的百分率均数低于参考值; CD8+ T细胞的百分率高于参考值。CD8+ /CD28- T细胞(Ts)的百分率较参考值增高; 而CD8+ /CD28+ T细胞(Tc)的百分率较参考值降低, 其中有 39例Tc细胞的百分率降低, 有 48例Ts细胞的百分率升高。CD3+HLA DR+和CD3- /HLA DR+细胞的百分率高于正常参考值,增加的例数分别是 36例和 30例。TCRVβ24个亚家族中,TCRVβ14的表达最高, TCRVβ5. 3和 23的表达次之, 表现出TCRVβ呈优势表达。正常对照组TCRVβ24个亚家族中,只TCRVβ14的表达最高, 与SARS患者的结果相一致; 但TCRVβ24个亚家族的分布格局不一样。两组比较, Vβ1、Vβ5. 2、Vβ5. 3、Vβ7. 2、Vβ9、Vβ11、Vβ13. 1、Vβ13. 2、Vβ17、Vβ18、Vβ22和Vβ23表达有显著性差异, 康复期SARS患者组均高于正常对照组。同时, SARS患者激素用?
Objective: To study the functional status of lymphocyte and the expression of 24 T-cell subfamilies of Vβ in convalescent SARS patients after Integrative Chinese and Western medicine treatment. Methods: Flow cytometry was used to observe the activation status of T lymphocyte subsets, T and B cells and CD3 + and TCRVβ1, 2, 3, 4, 5. 1,5. 2,5 in convalescent patients of our hospital. 3,7. 1,7. 2,8,9,11,12,13. 1,13. 2,13. 6,14,16,17,18,20,21. 3,22 and 23 24 Subfamily expression. Results: The percentages of CD3 + and CD4 + T cells in T cell subsets were lower than the reference value; the percentage of CD8 + T cells was higher than the reference value. The percentages of CD8 + / CD28- T cells (Ts) were higher than the reference value. The percentage of CD8 + / CD28 + T cells (Tc) was lower than the reference values high. The percentage of CD3 + HLA DR + and CD3- / HLA DR + cells was higher than the normal reference values, with an increase of 36 and 30, respectively. TCRVβ24 subfamilies, TCRVβ14 expression highest, TCRVβ5.3 and 23 expression followed by TCRVβ showed the dominant expression. TCRVβ24 subfamilies of normal controls had the highest expression of TCRVβ14, which was consistent with the results of SARS patients. However, the distribution patterns of TCRVβ24 subfamilies were different. There were significant differences in the expressions of Vβ1, Vβ5.2, Vβ5.3, Vβ7.2, Vβ9, Vβ11, Vβ13.1, Vβ13.2, Vβ17, Vβ18, Vβ22 and Vβ23 in both groups In the normal control group. At the same time, the use of hormones in patients with SARS?