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目的探讨使用一种具有较高相对分子质量的荧光紊进行灌注造影,使小鼠视网膜新生血管模型眼底血管显影的可行性。方法向高氧诱导的视网膜新生血管模型小鼠心脏灌注具有较高相对分子质量的荧光素,眼球经过短暂固定后,通过显微手术镜游离视网膜并铺片,在荧光显微镜下观察视网膜血管的分布与形态。结果在荧光显微镜下清楚地观察到全视网膜血管形态、走行;通过调节焦点。使视网膜浅层、深层以及二者交通支都清晰显影。高氧诱导的视网膜新生血管主要发生在视网膜有血管区与无血管区的交界部位,可见渗出、出血、微血管瘤的表现。方法简便、快捷,重复性好。结论通过这种方法可以清晰、准确地动态了解视网膜新生血管模型小鼠眼底血管的病变部位及形态学改变。采用此种具有较高相对分子质量的荧光素进行灌注造影可以较好地应用于视网膜新生血管性疾病的基础研究。
Objective To explore the feasibility of perfusing the retinal neovascularization model with retinal neovascularization using a fluorophore with high relative molecular mass. Methods The mouse model of retinal neovascularization induced by hyperoxia was perfused with fluorescein with high relative molecular mass. After a brief fixation, the eyeballs were detached from the retina by microscopic operating microscope and seeded. The distribution of retinal blood vessels was observed under a fluorescence microscope And form. Results The morphology of panretinal blood vessels was clearly observed under a fluorescence microscope; the focus was adjusted. Make the retina shallow, deep and both traffic branch clear development. Hyperoxia-induced retinal neovascularization mainly occurs at the border between the retinal vascular area and the non-vascular area, showing the appearance of exudation, hemorrhage and microangioma. Method is simple, fast, repeatable. Conclusion This method can clearly and accurately understand the retinal neovascularization retinal vascular lesions and morphological changes. Using this kind of fluorescein with higher relative molecular mass for perfusion imaging can be well applied to the basic research of retinal neovascular diseases.