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目的 :锌α2糖脂蛋白(zinc-alpha-2-glycoprotein 1,ZAG)是新近发现的具有复杂功能的蛋白,ZAG参与受精、脂代谢及免疫调节等多种细胞生物学行为,本研究探讨ZAG在大肠癌演进过程中的作用。方法:本研究构建ZAG干扰质粒,并转染入HT-29结肠癌细胞株,免疫印迹与逆转录聚合酶链式反应(RT-PCR)方法检测转染前后ZAG表达的变化;检测转染前后细胞三磷酸腺苷(adenosine triphosphate,ATP)含量的变化,以了解ZAG对细胞代谢的影响;利用小室实验检测转染前后HT-29细胞侵袭能力的变化。结果:ZAG干扰质粒成功转染入HT-29细胞并筛选出稳定转染细胞株;转染后的HT-29细胞ATP水平上调,且转染后的HT-29侵袭能力增强。结论:研究结果提示ZAG可能通过调节ATP水平增强大肠癌HT-29细胞的侵袭能力,为大肠癌治疗提供了有价值的新靶点。
AIM: Zinc is a recently discovered complex protein that is involved in various cellular biological processes such as fertilization, lipid metabolism and immunomodulation. In this study, ZAG Role in the evolution of colorectal cancer. METHODS: ZAG interference plasmids were constructed and transfected into HT-29 colon cancer cell lines. The changes of ZAG expression before and after transfection were detected by immunoblotting and reverse transcriptase-polymerase chain reaction (RT-PCR) The changes of adenosine triphosphate (ATP) content in cells were observed in order to understand the effect of ZAG on cell metabolism. The cell invasiveness of HT-29 cells before and after transfection was detected by the chamber assay. Results: The transfected HT-29 cells were transfected with HT-29 cells and successfully transfected with ZAG plasmid. The transfected HT-29 cells were up-regulated, and the invasion ability of HT-29 cells was enhanced. Conclusion: The results suggest that ZAG may enhance the invasion ability of HT-29 cells by regulating the level of ATP, which may provide a new target for the treatment of colorectal cancer.