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目的观察白细胞介素(IL)-10基因转染联合γ-干扰素(IFN-γ)对血管平滑肌细胞增殖的影响。方法感染复数(MOI)100的IL-10重组腺病毒(Ad/IL-10)转染人脐带动脉平滑肌细胞(HUASMCs),培养液加200U/ml的IFN-γ,噻唑蓝(MTT)比色法测定吸光度值(A_(570)值)。绘制生长曲线,流式细胞术分析细胞周期及凋亡,实时荧光定量聚合酶链反应(FQ-PCR)检测p21的表达。结果干预第6天,联合组、Ad/IL-10转染组、IFN-γ组的A_(570)值分别为1.342±0.051,1.523±0.012,1.559±0.027,联合组的增殖抑制作用大于单因素组．差异有统计学意义(P＜0.01)；联合抑制呈现时间依赖性；联合组的G_0/G_1期和凋亡细胞数增加,p_(21)表达上调。结论Ad/IL-10转染+IFN-γ是有意义的联合方案。
Objective To observe the effect of interleukin (IL) -10 gene transfection combined with interferon-γ (IFN-γ) on the proliferation of vascular smooth muscle cells. Methods Human umbilical cord artery smooth muscle cells (HUASMCs) were transfected with 100 recombinant adenovirus (MOI) of IL-10 at a concentration of 100U / ml. MTT colorimetric assay Determination of absorbance value (A_ (570) value). The growth curve was drawn, the cell cycle and apoptosis were analyzed by flow cytometry, and the expression of p21 was detected by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR). Results On the sixth day after intervention, the A 570 values in the combination group, Ad / IL-10 transfection group and IFN-γ group were 1.342 ± 0.051, 1.523 ± 0.012 and 1.559 ± 0.027, respectively. Factor group. The difference was statistically significant (P <0.01). The combined inhibition showed a time-dependent manner. The G 0 / G 1 phase and the number of apoptotic cells in the combination group were increased and the expression of p 21 was up-regulated. Conclusion Ad / IL-10 transfection + IFN-γ is a meaningful combination regimen.