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目的:探讨草棉花花瓣采用不同工艺提取的黄酮类化合物即提取物Ⅰ(FGF-Ⅰ)和提取物Ⅱ(FGF-Ⅱ)对D-氨基半乳糖(D-GalN)引起的实验性肝损伤的保护作用并比较2种提取物的作用强度,对药物筛选提供依据。方法:Wistar大鼠随机分为正常组、模型组、阳性对照组(联苯双酯组,150 mg.kg-1)、FGF-Ⅰ大、小剂量组(50,25 mg.kg-1)和FGF-Ⅱ大、小剂量组(50,25 mg.kg-1)7个组,腹腔注射350 mg.kg-1的D-GalN造成急性肝损伤动物模型;观察2种提取物对血清谷丙转氨酶(ALT)、谷草转氨酶(AST),肝组织匀浆超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-PX)活性等的影响。结果:对D-GalN引起的肝损伤动物模型FGF-Ⅰ和FGF-Ⅱ均能降低血清ALT,AST活性(P<0.01);FGF-Ⅰ大、小剂量组能增加肝组织SOD活性(P<0.01),大剂量可以降低MDA含量(P<0.05),但是对GSH-PX活性没有影响;FGF-Ⅱ大、小剂量组可以增加肝脏SOD,GSH-PX活性(P<0.05)并可以降低MDA含量(P<0.05)。结论:FGF-Ⅰ和FGF-Ⅱ均有一定的保肝作用,FGF-Ⅱ的作用优于FGF-Ⅰ。
OBJECTIVE: To investigate the effects of extracts of flavonoids extracted from petals of cotton (Gossypium hirsutum L.) with extracts I (FGF-I) and II (FGF-II) on experimental liver injury induced by D-galactosamine (D-GalN). The protective effect and the strength of the two extracts were compared to provide a basis for drug screening. Methods: Wistar rats were randomly divided into normal group, model group, positive control group (biphenyl-diester group, 150 mg.kg-1), FGF-I large and small dose group (50,25 mg.kg-1). In the 7 groups of large and small doses of FGF-II (50,25 mg.kg-1), an animal model of acute liver injury was induced by intraperitoneal injection of D-GalN at 350 mg.kg-1; two extracts were observed in the serum Alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD) activity in liver homogenate, malondialdehyde (MDA) content, glutathione peroxidase (GSH-PX) activity, etc. influences. RESULTS: FGF-I and FGF-II in the animal models of liver injury induced by D-GalN all reduced serum ALT and AST activities (P<0.01). Large and small doses of FGF-I increased liver SOD activity (P< 0.01), large doses can reduce MDA content (P <0.05), but have no effect on GSH-PX activity; FGF-II large and small dose groups can increase liver SOD, GSH-PX activity (P <0.05) and can reduce MDA Content (P<0.05). CONCLUSION: Both FGF-I and FGF-II have a certain hepatoprotective effect, and FGF-II is more effective than FGF-I.