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对采自浙江东阳市的玉米细菌性叶斑病标样进行分离和鉴定。结果表明,菌株YB125-2、YB125-3和YB184具有致病性。对这3个菌株进行培养、形态特征鉴别、常规生理生化性状测定、全细胞脂肪酸分析和16S rDNA序列分析的多种鉴定,鉴定为巨大芽孢杆菌Bacillus megaterium。巨大芽孢杆菌能够侵染玉米并引起叶斑病。
Isolation and identification of corn bacterial leaf spot samples collected from Dongyang, Zhejiang Province. The results showed that the strains YB125-2, YB125-3 and YB184 have pathogenicity. The three strains were identified as Bacillus megaterium by culturing, morphological characterization, routine physiological and biochemical characterization, whole cell fatty acid analysis and 16S rDNA sequence analysis. Bacillus megaterium infects corn and causes leaf spot.