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目的通过动物实验研究探索肝脏肿瘤微波治疗后的残留肿瘤的生物学特性的变化规律,借此评价非完全性微波治疗的效果,并为治疗后的复查和再次治疗时机提供科学依据。方法建立小鼠微波治疗后肝脏残余肿瘤模型,并分时段检测肿瘤细胞的增殖细胞核抗原和基质金属蛋白酶,并将治疗前后的结果进行比较,观察其生物学特性的变化及与对照组小鼠的生存时间及肺部转移情况。结果PCNA、MMP-9在微波治疗后两项表达均呈一过性的降低。微波治疗组小鼠与对照组相比生存期平均延长57%,肺部转移率低于对照组。结论微波治疗后残留肿瘤细胞的增殖活性和侵袭能力在短期内有明显的降低,残留肿瘤生长和转移受到一定程度的抑制,肿瘤分化程度较治疗前有一定的改善,微波治疗即使不能一次灭活肿瘤,但在一定程度上可延长机体的生存时间。
OBJECTIVE: To investigate the variation of biological characteristics of residual tumor after microwave treatment of liver tumors through animal experiments to evaluate the effect of incomplete microwave therapy and to provide a scientific basis for the post-treatment review and re-treatment. Methods The model of residual liver tumor was established after microwave treatment in mice. The proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase (TIMP-1) in tumor cells were detected at different time points. The changes of biological characteristics before and after treatment were observed and compared with those of control mice Survival time and lung metastasis. Results Both expression of PCNA and MMP-9 showed a transient decrease after microwave treatment. Compared with the control group, mice in the microwave treatment group had an average survival of 57% longer than that of the control group. The lung metastasis rate was lower in the microwave treatment group than in the control group. Conclusion The proliferation activity and invasion ability of residual tumor cells after microwave treatment decreased obviously in a short period of time, and the growth and metastasis of residual tumor were inhibited to a certain extent. The degree of tumor differentiation was improved to a certain extent before treatment, and microwave treatment could not be inactivated once Tumor, but to a certain extent, can extend the body’s survival time.