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目的 探讨左旋四氢巴马汀 (L -THP)对缺氧 复氧时脑微血管内皮细胞细胞间粘附分子 1(ICAM 1)表达的影响。方法 采用流式细胞仪检测脑微血管内皮细胞在缺氧、缺氧 复氧及加入L -THP后ICAM 1的阳性细胞百分率。结果 缺氧 12h ,脑微血管内皮细胞ICAM 1的表达与常氧时差异无显著意义 ,缺氧 12h再给氧 12h ,ICAM 1的表达明显增加 ,加入L -THP后 ,ICAM - 1的阳性细胞百分率明显降低。结论 L -THP可减少缺氧 复氧时脑微血管内皮细胞ICAM - 1的表达
Objective To investigate the effect of L-THP on the expression of ICAM-1 in hypoxia-reoxygenation brain microvascular endothelial cells. Methods Flow cytometry was used to detect the percentage of ICAM-1 positive cells in hypoxic, hypoxia-reoxygenation and L-THP groups. Results After hypoxia for 12 hours, the expression of ICAM - 1 in cerebral microvascular endothelial cells was not significantly different from that in normoxia. The expression of ICAM - 1 was significantly increased in hypoxia for 12 hours and then in oxygen for 12 hours. After addition of L - THP, the percentage of ICAM - 1 positive cells Obvious reduction. Conclusion L-THP can reduce the expression of ICAM - 1 in cerebral microvascular endothelial cells during anoxia - reoxygenation