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目的探讨 Creutzfeldt-Jakob 病(CJD)脑组织内14-3-3蛋白表达及其诊断意义。方法实验组为5例 CJD 额叶组织,对照组为4例非 CJD 额叶组织。采用14-3-3β和ε抗体,通过链霉菌抗生物素蛋白-过氧化酶(S-P)免疫组织化学方法进行检测。并参照 KB、胶质纤维酸性蛋白(GFAP)、PrP 免疫组织化学染色和129基因型检测结果。结果实验组5例脑组织14-3-3蛋白有较多地表达,其中3例灰质与星形胶质细胞尤著。其表达程度与 PrP 沉积类型有关,而与 PrP129基因型无关。对照组4例中除2例急性脑挫伤仅个别神经细胞有表达外,肾上腺脑白质营养不良与额颞叶痴呆灰质和白质均无表达。结论 14-3-3蛋白在脑内较多地表达,有利于 CJD 的病理诊断。
Objective To investigate the expression of 14-3-3 protein in Creutzfeldt-Jakob disease (CJD) brain tissue and its diagnostic significance. Methods The experimental group consisted of 5 CJD frontal lobe tissues and 4 control non-CJD frontal lobe tissues. Detection was performed by streptavidin-peroxidase (S-P) immunohistochemistry using 14-3-3 [beta] and [epsilon] antibodies. And KB, glial fibrillary acidic protein (GFAP), PrP immunohistochemical staining and 129 genotype detection results. Results In the experimental group, the expression of 14-3-3 protein in brain tissue was more in 5 cases, especially in gray matter and astrocyte in 3 cases. Its expression level is related to the type of PrP deposition, but not to the PrP129 genotype. In the control group, the expression of adrenoleukodystrophy and frontotemporal dementia gray matter and white matter were all not seen except for the expression of some nerve cells in 2 cases of acute cerebral contusion. Conclusions 14-3-3 protein is more expressed in the brain, which is beneficial to the pathological diagnosis of CJD.