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目的:探讨他汀类药物对β淀粉样多肽(Aβ)生成的影响。方法:将人神经母细胞瘤SH-SY5Y细胞分为对照组、转染组、阿托伐他汀干预组和辛伐他汀干预组,对照组不予任何处理,其它3组通过pcDNA3.0-YFP-C99质粒转染构建阿尔茨海默病(AD)细胞模型,阿托伐他汀干预组和辛伐他汀干预组分别加入不同浓度(5、10、20、50、100μmol/L)的阿托伐他汀、辛伐他汀。4组均在培养前和培养12、24、48、72h时,通过荧光显微镜定性观察,ELISA和Western blot定量分析细胞内Aβ的变化。结果:荧光显微镜下观察他汀类药物能显著减少细胞内Aβ聚集。不同浓度的他汀类药物和细胞培养时间均能减少Aβ,呈浓度-时间依赖性。他汀类药物浓度为20μmol/L和细胞培养24h时,各组间的Aβ减少有统计学意义(P<0.05)。结论:不同种类的他汀类药物能减少细胞内Aβ聚集,并且具有浓度-时间依赖性。
Objective: To investigate the effect of statins on the production of β-amyloid polypeptide (Aβ). Methods: Human neuroblastoma SH-SY5Y cells were divided into control group, transfection group, atorvastatin intervention group and simvastatin intervention group, the control group without any treatment, the other three groups by pcDNA3.0-YFP C99 plasmid was used to construct Alzheimer’s disease (AD) cell model. Atorvastatin intervention group and simvastatin intervention group were treated with atorvastatin (5,10,20,50,100μmol / L) Statin, simvastatin. 4 groups were cultured before and 12,24,48,72 h, qualitative observation by fluorescence microscopy, ELISA and Western blot quantitative analysis of intracellular Aβ changes. Results: Observing statins under fluorescent microscope can significantly reduce intracellular Aβ aggregation. Different concentrations of statins and cell culture time can reduce Aβ, in a concentration-time-dependent manner. Statin drug concentration of 20μmol / L and cell culture 24h, the Aβ decreased between groups was statistically significant (P <0.05). CONCLUSIONS: Different classes of statins reduce intracellular Aβ aggregation in a time-and concentration-dependent manner.