目的探讨吸入中浓度氧新生小鼠血清和肺部血管内皮细胞膜抗原CD105表达水平及意义,寻求高氧肺损伤新生小鼠肺微血管发育可能的机制。方法清洁级4日龄昆明小鼠50只,随机分为观察组、对照组各25只。观察组置于氧箱中(FiO2:0.6),对照组置于空气中(FiO2:0.21),建立高氧肺损伤小鼠模型,每组分别于实验第0(实验开始时)、7、14、21、28天时随机选取5只小鼠留取血标本及肺组织,HE染色观察肺组织病理形态,酶联免疫吸附法检测血中CD105含量,免疫组化染色法检测肺组织CD105表达水平,并分析血清CD105浓度与肺组织CD105表达量的相关性。结果观察组HE染色下正常肺泡结构消失、肺泡融合、肺泡间隔增厚,肺泡炎和肺组织纤维化增加,放射状肺泡计数较对照组明显减少(P<0.01),随着吸氧时间延长,CD105的表达水平呈逐渐增高趋势,实验第7、14、21、28天观察组血清CD105浓度和肺组织CD105表达水平均高于对照组,血清(ng/L):[7天:(346.4±14.7)比(265.7±2.0),14天:(400.2±20.1)比(266.3±3.2),21天:(505.1±6.1)比(267.1±5.8),28天:(451.9±10.0)比(268.6±4.5),P<0.01];肺组织累积光密度值:[7天:(2.24±0.15)比(1.19±0.14),14天:(3.42±0.20)比(1.20±0.11),21天:(4.35±0.18)比(1.16±0.18),28天:(4.04±0.12)比(1.17±0.14),P<0.01],且观察组CD105在血清中与肺组织中的表达水平呈正相关(r=0.973,P<0.001)。结论 CD105可能代替传统的血管内皮生长因子和血管生成素-1,成为氧疗通气诱导血管重塑的重要血管生长因子。 Objective To investigate the expression and significance of membrane antigen CD105 in serum and pulmonary vascular endothelial cells of neonatal rats exposed to medium concentration oxygen and to explore the possible mechanism of lung microvascular development in neonatal mice with hyperoxia-induced lung injury. Methods 50 Kunming mice, 4 days old, were randomly divided into observation group (n = 25) and control group (n = 25). The rats in the observation group were placed in an oxygen chamber (FiO2: 0.6), and the control group was placed in the air (FiO2: 0.21) to establish a mouse model of hyperoxia-induced lung injury. , 21,28 days were randomly selected 5 mice to take blood samples and lung tissue, HE staining to observe the pathological changes of lung tissue, serum CD105 levels by enzyme-linked immunosorbent assay, immunohistochemical staining of lung tissue CD105 expression levels, The correlation between serum CD105 concentration and expression of CD105 in lung tissue was analyzed. Results In the observation group, the normal alveolar structure disappeared, the alveolar fusion, thickening of alveolar septa, alveolitis and lung fibrosis increased, and the number of radial alveolar was significantly decreased (P <0.01). With the increase of oxygen inhalation time, CD105 The level of CD105 in lung tissue and the level of CD105 expression in the observation group were higher than those in the control group on the 7th, 14th, 21st, the 28th day (ng / L): [7 days: (346.4 ± 14.7 ) Ratio (265.7 ± 2.0), 14 days: (400.2 ± 20.1) vs (266.3 ± 3.2), 21 days: (505.1 ± 6.1) vs (267.1 ± 5.8), 28 days: (451.9 ± 10.0) vs 4.5), P <0.01]. The cumulative optical density values ​​of lung tissue were: (7 days: (2.24 ± 0.15) vs 1.19 ± 0.14, 14 days: (3.42 ± 0.20) vs 4.35 ± 0.18 vs 1.16 ± 0.18, 28 days: (4.04 ± 0.12) vs (1.17 ± 0.14), P <0.01], and there was a positive correlation between the level of CD105 in the serum and lung tissue (r = 0.973, P <0.001). Conclusion CD105 may replace traditional vascular endothelial growth factor and angiopoietin-1 and become an important angiogenic factor for angiogenesis induced by oxygen therapy.