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目的通过克隆凝血因子C同源物基因(Coagulation factor C homology,COCH)全长cDNA,为COCH编码蛋白cochlin的功能研究打下基础。方法从听力正常人新鲜外周静脉血中提取总RNA,应用一步法RT-PCR试剂盒进行COCH反转录,转录产物进行浓缩、酶切、连接。结果反转录COCH cDNA全长与标准基因序列比较,结果完全相符,得到完整的COCH cDNA全长序列。结论本研究成功克隆了COCH cDNA全长序列,为COCH编码蛋白cochlin的功能研究打下了良好的基础。
Objective To clone the full length cDNA of COCH gene and lay a foundation for the functional study of COCH protein cochlin. Methods Total RNA was extracted from fresh peripheral venous blood of normal people. COCH reverse transcription was performed by one-step RT-PCR kit. The transcripts were concentrated, digested and ligated. Results The full-length COCH cDNA of reverse transcription was compared with the standard gene sequences. Conclusion The full length cDNA of COCH cDNA was successfully cloned in this study, which laid a good foundation for the study of the function of COCH encoding protein cochlin.