论文部分内容阅读
目的探讨人端粒酶RNA(hTERC)及原癌基因C-MYC在新疆维吾尔族妇女宫颈病变筛查中的临床应用价值。方法收集1 000例新疆维吾尔族妇女的宫颈脱落细胞,均行宫颈液基薄层细胞学(TCT)、高危型HPV筛查(HR-HPV)及荧光原位杂交技术(FISH)检测hTERC和C-MYC基因,任一指标阳性者行阴道镜下活检,分析hTERC、C-MYC基因在细胞学及病理学分级中的表达水平,ROC曲线分析hTERC和C-MYC基因单一和联合检测对高级别宫颈病变(≥CINⅡ期)的临床诊断效能。结果hTERC、C-MYC基因表达水平均随宫颈病变程度的升高而升高。hTERC基因单独诊断宫颈高级别病变(≥CINⅡ期)的ROC曲线下面积(AUCROC)为0.879,C-MYC基因为0.835,两者联合检测为0.904,且两者联合检测的敏感性(90.77%)、阴性预测值(96.00%)和准确性(90.22%)均高于单一指标检测。结论 hTERC、C-MYC基因可作为临床宫颈病变高级别筛查的指标。
Objective To investigate the clinical value of human telomerase RNA (hTERC) and proto-oncogene C-MYC in the screening of Uygur women with cervical lesions in Xinjiang. Methods Cervical exfoliated cells from 1000 Uygur women in Xinjiang were collected and tested for TCT, HR-HPV and FISH. -MYC gene, and colposcopy biopsy was performed in any of the indicators. The expression levels of hTERC and C-MYC in cytology and pathological grade were analyzed. Single and combined detection of hTERC and C-MYC genes by ROC curve analysis was performed on high-grade Cervical lesions (≥ CIN Ⅱ) clinical diagnostic efficacy. Results The expression levels of hTERC and C-MYC increased with the increase of cervical lesions. The area under the ROC curve (AUCROC) was 0.879, the C-MYC gene was 0.835, and the combined detection of the two was 0.904 (90.77%) for the diagnosis of high grade cervical lesions (≥CINⅡ) , Negative predictive value (96.00%) and accuracy (90.22%) were higher than a single index test. Conclusion The hTERC and C-MYC genes can be used as a high-level screening index for clinical cervical lesions.