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目的探讨钙池操纵的钙通道(SOC)抑制剂(2APB)对大鼠肝细胞SOC电流(Isoc)的影响。方法急性分离大鼠肝细胞,应用全细胞膜片钳记录技术测定大鼠肝细胞Isoc,并观察2APB对该电流的影响。结果在急性分离的大鼠肝细胞记录到Isoc,从其电流电压(IV)曲线可以看出该Isoc的反转电位为-2.5mV左右。在钳制电位为-100mV时,该Isoc为(-664.52±140.44)pA(n=8);20、40、60、80、100μmol/L的2APB分别使Isoc由给药前的(-664.52±140.44)pA下降至给药后的(-564.80±111.01)、(-490.11±73.97)、(-362.01±55.91)、(-295.64±50.03)、(-232.12±46.47)pA,给药前后比较差异有统计学意义(P<0.05或P<0.01);2-APB的抑制作用呈浓度依赖性增强,其EC50为(64.63±10.56)μmol/L。结论2APB对急性分离的大鼠肝细胞Isoc具有显著的抑制作用,这对于进一步深入进行肝细胞钙超载的发生机制和防治具有重要意义。
Objective To investigate the effect of calcium channel (SOC) inhibitor (2APB) administered by calcium channel on SOC in rat hepatocytes (Isoc). Methods Acute isolation of rat hepatocytes was performed using whole-cell patch clamp recording technique to determine Isoc in rat hepatocytes. The effect of 2APB on this current was also observed. Results Isoc was recorded in acutely isolated rat hepatocytes, and its Isoc reversal potential was about -2.5 mV from its current voltage (IV) curve. Isoc was (-664.52 ± 140.44) pA (n = 8) at clamp potential -100 mV; 2 APB at 20, 40, 60, 80 and 100 μmol / L caused Isoc to increase from (-664.52 ± 140.44 ) pA decreased to (-564.80 ± 111.01), (-490.11 ± 73.97), (-362.01 ± 55.91), (-295.64 ± 50.03) and (-322.12 ± 46.47) pA after administration Statistically significant (P <0.05 or P <0.01). The inhibitory effect of 2-APB was enhanced in a concentration-dependent manner with an EC50 of (64.63 ± 10.56) μmol / L. Conclusions 2APB has a significant inhibitory effect on acutely isolated Isoc in rat hepatocytes, which is of great significance for the further study on the mechanism and prevention and treatment of hepatocellular calcium overload.