The rice-wheat rotation in southern China is characterized by frequent flooding-draining water regime and heavy nitrogen(N)fertilization. There is a substantial lack of studies into the behavior of dissolved organic nitrogen(DON) in the intensively managed agroecosystem. A 3-year in situ field experiment was conducted to determine DON leaching and its seasonal and yearly variations as affected by fertilization, irrigation and precipitation over 6 consecutive rice/wheat seasons. Under the conventional N practice(300kg N ha-1for rice and 200 kg N ha-1for wheat), the seasonal average DON concentrations in leachate(100 cm soil depth) for the three rice and wheat seasons were 0.6–1.1 and 0.1–2.3 mg N L-1, respectively. The cumulative DON leaching was estimated to be1.1–2.3 kg N ha-1for the rice seasons and 0.01–1.3 kg N ha-1for the wheat seasons, with an annual total of 1.1–3.6 kg N ha-1. In the rice seasons, N fertilizer had little effect(P > 0.05) on DON leaching; precipitation and irrigation imported 3.6–9.1 kg N ha-1of DON, which may thus conceal the fertilization effect on DON. In the wheat seasons, N fertilization had a positive effect(P < 0.01)on DON. Nevertheless, this promotive effect was strongly influenced by variable precipitation, which also carried 1.8–2.9 kg N ha-1of DON into fields. Despite a very small proportion to chemical N applied and large variations driven by water regime, DON leaching is necessarily involved in the integrated field N budget in the rice-wheat rotation due to its relatively greater amount compared to other natural ecosystems. The rice-wheat rotation in southern China is characterized by frequent flooding-draining water regime and heavy nitrogen (N) fertilization. There is a substantial lack of studies into the behavior of dissolved organic nitrogen (DON) in the intensively managed agroecosystem. A 3 -year in situ field experiment was conducted to determine DON leaching and its seasonal and yearly variations as affected by fertilization, irrigation and precipitation over 6 consecutive rice / wheat seasons. Under the conventional N practice (300 kg N ha-1 for rice and 200 kg N ha-1 for wheat, the seasonal average DON concentrations in leachate (100 cm soil depth) for the three rice and wheat seasons were 0.6-1.1 and 0.1-2.3 mg N L-1, respectively. .1-2.3 kg N ha-1for the rice seasons and 0.01-1.3 kg N ha-1for the wheat seasons, with an annual total of 1.1-3.6 kg N ha-1. In the rice seasons, N fertilizer had little effect ( P> 0.05) on DON leaching; precipitation and irrigation was 3.6-9.1 kg N ha-1 of DON, which may therefore have conceal the fertilization effect on DON. In the wheat seasons, N fertilization had a positive effect (P <0.01) on DON. Nevertheless, this promotive effect was strongly influenced by variable precipitation, which also carried 1.8-2.9 kg N ha-1 of DON into fields. Despite a very small proportion to chemical N applied and large variations driven by water regime, DON leaching is necessarily involved in the integrated field N budget in the rice -wheat rotation due to its relatively greater amount than to other natural ecosystems.