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目的观察在百草枯(PQ)致大鼠肺纤维化模型中肺组织胎盘生长因子(PLGF)的变化。方法将42只健康成年雌性SD大鼠随机分为对照组和染毒组,每组21只,分别观察7、14和28 d。染毒组于实验开始一次性灌胃染毒PQ(40 mg/kg),对照组予以等量的生理盐水。取肺组织行半定量的病理组织学检查、羟脯氨酸测定,并利用半定量逆转录-聚合酶链反应(RT-PCR)和免疫组化方法测定PLGF mRNA和蛋白表达。结果PQ染毒后大鼠肺泡炎、肺纤维化程度积分明显增高,肺组织羟脯氨酸含量明显高于对照组,差异有统计学意义(P<0.01)。7、14、28 d时肺组织PLGF mRNA表达分别1.28±0.29、0.80±0.07、0.65±0.13,均高于对照组,差异有统计学意义(P<0.01);免疫组化阳性指数在3个时点分别为2.27±0.34、1.78±0.41、1.25±0.69,明显高于对照组,差异有统计学意义(P<0.01)。结论PQ中毒大鼠肺组织PLGF mRNA和蛋白水平的表达明显上调。
Objective To observe the changes of lung tissue placental growth factor (PLGF) in paraquat (PQ) -induced pulmonary fibrosis in rats. Methods Forty-two healthy adult female Sprague-Dawley rats were randomly divided into control group and exposure group with 21 rats in each group, which were observed for 7, 14 and 28 days respectively. At the beginning of the experiment, the rats in the exposure group were treated with PQ (40 mg / kg) by intragastric administration, and the control group received the same amount of normal saline. Semiquantitative histopathological examination and hydroxyproline determination were performed on the lung tissue. PLGF mRNA and protein expression were determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Results After the PQ exposure, the alveolitis and the degree of pulmonary fibrosis in rats were significantly increased. The content of hydroxyproline in lung tissue was significantly higher than that in the control group (P <0.01). The expression of PLGF mRNA in lung tissue at 7, 14 and 28 d were 1.28 ± 0.29, 0.80 ± 0.07 and 0.65 ± 0.13, respectively, which were significantly higher than those in control group (P <0.01). The immunohistochemical positive index was 3 The time points were 2.27 ± 0.34, 1.78 ± 0.41 and 1.25 ± 0.69, respectively, which were significantly higher than those in the control group (P <0.01). Conclusion The expression of PLGF mRNA and protein in lung tissue of PQ poisoned rats was significantly up-regulated.