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目的 :研究视网膜缺血组织中线粒体表面积密度的变化特点。方法 :给SD大鼠球后注射去甲肾上腺素 ,建造视网膜缺血的动物模型 ;用电镜和图像分析等方法 ,对缺血视网膜组织中线粒体表面积密度的动态改变进行体视学定量分析。结果 :大鼠球后注射去甲肾上腺素后 ,视网膜内节、外网状层及内网状层在缺血状态下 ,线粒体空泡的表面积密度增高 ,尤以内网状层表现最明显 ,并随着时间变化有显著性差异 ,呈现缺血→损伤→修复的过程。结论 :球后注射去甲肾上腺素后 ,视网膜线粒体的表面积密度发生缺血性改变 ;缺血视网膜组织不同部位的损伤 ,其线粒体的表面积密度改变不同 ;线粒体对缺血极为敏感 ,反应最强烈。
Objective: To study the changes of mitochondrial surface area density in retinal ischemic tissue. Methods: SD rats were injected with norepinephrine to create a model of retinal ischemia. The dynamic changes of mitochondrial surface area density in ischemic retinal tissue were analyzed stereoscopically by electron microscopy and image analysis. RESULTS: After injection of noradrenaline into the posterior globe, the surface area density of mitochondrial vacuoles increased in the inner retinal ganglion, the outer reticular layer and the inner reticular layer, especially in the reticular layer With the time changes have significant differences, showing the process of ischemia → injury → repair. CONCLUSION: After noradrenal injection, the surface area density of retinal mitochondria undergoes ischemic changes. The damage of different parts of ischemic retinal tissue changes the surface area density of mitochondria. The mitochondria are extremely sensitive to ischemia and the strongest reaction.