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采用酶联免疫法 (ELISA)及薄层 -荧光测定法分析了僵藕生长过程中茎和叶中内源激素和多胺含量的变化。结果表明 :僵藕茎、叶中IAA、GA1+3和ZRS 水平一般都明显低于正常藕 ,而ABA水平则一直明显高于正常藕 ,这可能是僵藕生长势弱的原因所在 ;生长后期 ,正常藕茎、叶中IAA水平成倍提高 ,ABA维持低水平 ,而僵藕则IAA维持低水平 ,ABA迅速上升 ,这可能是僵藕早衰的生理原因。内源精胺 (Spm)、腐胺 (Put)和尸胺 (Cad)都普遍高于正常藕 ,且变化幅度大于正常藕 ,提示了内源多胺与僵藕的发生有密切关系。
The changes of endogenous hormones and polyamines in the stems and leaves during the growth of Ziziphus jujuba were analyzed by enzyme-linked immunosorbent assay (ELISA) and thin layer-fluorescence assay. The results showed that the levels of IAA, GA1 + 3 and ZRS in the leaves and stems of Ziziphus jujuba Mill. Were generally lower than those in normal lotus plants, while the level of ABA was always significantly higher than that in normal lotus plants. In the normal lotus root, the level of IAA in the leaves increased exponentially, the level of ABA remained low, while the level of IAA in sturdy lotus root kept low and ABA rose rapidly, which may be the physiological reason for the premature decline of stubble. Endogenous spermine (Spm), putrescine and cadaverine (Cad) were generally higher than the normal lotus root, and the magnitude of change is greater than the normal lotus root, suggesting that endogenous polyamines is closely related to the occurrence of sturgeon lotus root.