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目的随访监测确诊SARS患者,研究SARS-CoV、新型呼肠病毒和脊髓灰质炎病毒在患者体内的存在状况及与SARS的可能相关性。方法采集8例SARS患者痊愈2年后的咽拭子、唾液(或痰液)、尿液、血清及连续3天的粪便标本,用RT-PCR的方法分别扩增SARS-CoV、呼肠病毒和脊髓灰质炎病毒;采用ELISA的方法检测患者血清中的SARS-CoV抗体和脊髓灰质炎病毒IgG抗体。结果8例患者中有4例的粪便中脊髓灰质炎病毒RNA阳性,均为Sabin1毒株,在5′端非编码区的第480nt及VP1蛋白编码区的第2795nt两个重要的减毒位点上均发生了回复突变;SARS-CoV和新型呼肠病毒均阴性。对照组标本中均未检测到上述三种病毒。3例随访患者血清中SARS-CoV抗体阳性;4例患者血清中脊髓灰质炎IgG抗体阳性。25例健康体检者的血清中SARS-CoV抗体均阴性,其中23例的血清中脊髓灰质炎病毒抗体阳性。结论SARS患者痊愈2年后的血清中特异性脊髓灰质炎病毒抗体与健康人有显著差异,其粪便标本中仍有较高的脊髓灰质炎病毒阳性率,而且病毒核酸在两个重要的减毒位点上均发生了回复突变。
Objective To investigate the presence of SARS-CoV, novel reovirus and poliovirus in patients with SARS and their possible relationship with SARS. Methods Throat swabs, saliva (or sputum), urine, serum and stool samples from 8 SARS patients recovered after 2 years were collected. The stool samples of SARS-CoV, reovirus And poliovirus. Serum samples of SARS-CoV and poliovirus IgG were detected by ELISA. Results Four out of eight patients were positive for poliovirus RNA in the faeces, all of which were Sabin1 strains. They were found in the 480nt of the 5 ’non-coding region and 2795nt of the VP1 protein coding region of the two important attenuating sites All of them had a back mutation; SARS-CoV and new reovirus were negative. The three samples were not detected in the control group. Serum SARS-CoV antibody was positive in 3 cases and 4 cases were positive for poliomyelitis IgG antibody. Serum samples from 25 healthy volunteers were all negative for SARS-CoV antibodies, of which 23 were seropositive for poliovirus antibodies. Conclusion Serum specific poliovirus antibodies in SARS patients after two years of recovery have significant differences with healthy people, and the stool samples still have a high positive rate of poliovirus, and the viral nucleic acids are attenuated in two important The site of the mutation occurred on the back.