【摘 要】
：
目的 探讨小分子NEK2干扰RNA (siRNA-NEK2)逆转人肺癌耐药细胞(A549/DDP)耐药性的研究.方法 应用Real-time PCR法及Western blot法验证NEK2基因在人肺腺癌耐药细胞系(A549/DD
【机 构】
：
第三军医大学西南医院呼吸科,解放军75600部队医院接诊室
论文部分内容阅读
目的 探讨小分子NEK2干扰RNA (siRNA-NEK2)逆转人肺癌耐药细胞(A549/DDP)耐药性的研究.方法 应用Real-time PCR法及Western blot法验证NEK2基因在人肺腺癌耐药细胞系(A549/DDP)细胞系呈现高表达;并采用脂质体为转染介质,将NEK2小片段RNA导入A549/DDP细胞沉默NEK2基因;应用MTT法观察顺铂、阿霉素对沉默NEK2基因后的A549/DDP细胞的细胞毒活性变化.结果 NEK2-mRNA、NEK2蛋白在A549/DDP细胞系表达水平均显著高于A549细胞系,差异有统计学意义(P＜0.05);顺铂、阿霉素对A549/DDP细胞的半数抑制浓度(IC50)分别是(53.08±0.56)、(8.09±0.31) μg/mL,对siRNA-control A549/DDP细胞的IC50分别是(53.09±0.78)、(8.10±0.98) μg/mL,而对siRNA-NEK2 A549/DDP细胞的1C50有显著下降,其值分别是(18.59±0.10)、(2.30±0.14) μg/mL,两组比较,差异均有统计学意义(P＜0.05).结论 小分子NEK2干扰RNA沉默NEK2可以提高耐药的肺癌细胞对化疗药物的敏感性.NEK2可能成为逆转肺癌耐药的一个新的靶点.
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