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目的 探讨宁夏固原地区结核分子流行病学的特点。方法 提取结核分枝杆菌基因组DNA ,经限制性内切酶PvuⅡ切割、琼脂糖凝胶电泳和Southern转印后 ,用荧光标记的IS6 110DNA序列中 2 45bp探针杂交 ,以核酸化学发光试剂盒探测荧光信号 ,比较各菌株指纹的IS6 110拷贝数和带型 ,分析流行菌株的特点。结果 固原地区流行结核分枝杆菌临床分离株DNA指纹图谱带型相似 ,IS6 110拷贝数较多。结论 固原地区流行的结核分枝杆菌多态性程度较低 ,在基因上具较近的亲缘关系。
Objective To explore the molecular epidemiology of tuberculosis in Guyuan area of Ningxia. Methods Mycobacterium tuberculosis genomic DNA was extracted and subjected to restriction endonuclease cleavage with PvuII, agarose gel electrophoresis and Southern blotting. After hybridization with a 2 45 bp probe in a fluorescently labeled IS6 110 DNA sequence, the probe was probed with a nucleic acid chemiluminescence kit Fluorescence signals were compared with the IS6 110 copy number and band pattern of each strain fingerprinting to analyze the characteristics of the epidemic strains. Results The genotypes of DNA fingerprinting of clinical isolates of Mycobacterium tuberculosis from Guyuan were similar, and IS6 110 had more copies. Conclusion The prevalence of Mycobacterium tuberculosis in the Guyuan area is relatively low, and it is closely related to the gene.