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目的:建立增乳膏中芍药苷的含量测定方法。方法:高效液相色谱法测定增乳膏中芍药苷的含量。选用Ultimate~(TM)XB-C_(18)色谱柱(250mm×4.6mm,5μm);乙腈-0.1%磷酸(14:86)为流动相;流速0.8ml·min~(-1)检测波长230nm。结果:芍药苷在0.10~1.97μg范围内线性关系良好(r=0.999 8),平均回收率为100.2%,RSD 0.7%(n=6)。结论:方法简便,快速,准确,可用于增乳膏中芍药苷的含量测定。
Objective: To establish a method for determination of paeoniflorin in Zeng cream. Methods: HPLC method was used to determine the content of paeoniflorin in Zeng cream. The Ultimate(TM) XB-C_(18) column (250mm×4.6mm, 5μm) was used. Acetonitrile-0.1% phosphoric acid (14:86) was used as the mobile phase. The flow rate was 0.8ml·min-1 and the detection wavelength was 230nm. . Results: There was a good linearity in the range of 0.10~1.97μg (r=0.999 8). The average recovery was 100.2% and the RSD was 0.7% (n=6). Conclusion: The method is simple, rapid and accurate. It can be used to determine the content of paeoniflorin in the “Zeng cream”.