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目的探讨厄多司坦对大鼠睾丸扭转/复位模型的保护作用。方法成年雄性大鼠32只,体重(200±20)g,随机分为4组,每组8只,分别为:假手术组,扭转复位组,扭转复位+生理盐水组,扭转复位+厄多司坦组。按Turner法建立睾丸扭转模型,扭转复位术后24h切取双侧睾丸分别留取组织匀浆和石蜡切片的标本待检测。TUNEL法测定凋亡细胞,化学比色法测定SOD及MDA含量。结果与扭转复位组相比,厄多司坦组生精细胞凋亡明显减少,超氧化物歧化酶活性上升,丙二醛含量下降,其差异有显著性。结论厄多司坦对大鼠单侧睾丸扭转后双侧睾丸生精功能具有保护作用
Objective To investigate the protective effect of erdosteine on testicular torsion / detorsion in rats. Methods Thirty-two adult male rats weighing 200 ± 20 g were randomly divided into 4 groups with 8 rats in each group: sham operation group, reverse rotation reduction group, reverse rotation reduction + saline group, reverse rotation reduction + Secretary Tanzania group. The testicular torsion model was established by Turner’s method. Twenty-four hours after the torsion reduction, the bilateral testis was excised and the specimens of tissue homogenate and paraffin sections were collected for testing. Apoptotic cells were detected by TUNEL method and SOD and MDA contents were determined by chemical colorimetry. Results The apoptosis of spermatogenic cells was significantly decreased, the activity of superoxide dismutase (SOD) increased and the content of malondialdehyde (MDA) decreased in Erdosteine group compared with that in the torsion reduction group. The difference was significant. Conclusion Erdosteine has a protective effect on the spermatogenic function of bilateral testis after unilateral testicular torsion in rats