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目的 研制水痘IgG(VZV-IgG)酶联检测试剂盒。方法 将VZV接种二倍体细胞,收获病毒,经纯化后用作包被抗原;辣根过氧化物酶(HRP)标记羊抗人IgG制备酶联检测试剂盒并进行质量检定。结果 VZV-IgG酶联检测试剂盒敏感度与膜抗原荧光抗体法(FAMA)近似。结论 成功地制备了VZV-IgG酶联检测试剂盒。
Objective To develop varicella IgG (VZV-IgG) enzyme linked detection kit. Methods VZV was inoculated with diploid cells and harvested for virus purification. The purified antigen was then used as coating antigen. Horseradish peroxidase (HRP) labeled goat anti-human IgG was used to prepare the enzyme-linked immunosorbent assay kit for quality control. Results The sensitivity of VZV-IgG ELISA kit was similar to that of FAMA. Conclusion VZV-IgG enzyme linked detection kit was successfully prepared.