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目的研究常压氧疗对大鼠脑缺血再灌注后血脑屏障损伤的作用及机制。方法采用大鼠大脑中动脉线栓法制备脑缺血再灌注模型。随机将30只SD雄性大鼠,分为模型对照组和常压氧疗治疗组各15只,缺血90min后再灌注24h。采用TTC染色、伊文思蓝法和明胶酶谱技术,分别检测脑梗死体积、血脑屏障通透性及缺血脑组织基质金属蛋白酶-9(MMP-9)的活性,并进行神经功能评分。结果与对照组比较,常压氧疗治疗组大鼠的脑梗死体积、缺血脑组织伊文思蓝外渗率及MMP-9的活性显著降低(P(0.01),神经功能缺陷也得到显著改善。结论缺血期内的常压氧疗对大鼠脑缺血再灌注后血脑屏障的损伤具有保护作用,其作用机制可能是通过抑制MMP-9的活性来实现的。
Objective To investigate the effect and mechanism of atmospheric oxygen therapy on blood-brain barrier injury after cerebral ischemia-reperfusion in rats. Methods The cerebral ischemia reperfusion model was established by middle cerebral artery occlusion in rats. Thirty male Sprague-Dawley rats were randomly divided into model control group and normobaric oxygen therapy group (n = 15 each). After ischemia for 90 min, reperfusion was performed for 24 h. The volume of cerebral infarction, the permeability of the blood-brain barrier and the activity of matrix metalloproteinase-9 (MMP-9) in ischemic brain tissue were detected by TTC staining, Evans blue method and gelatin zymography, respectively. Neurological scores were also evaluated. Results Compared with the control group, the volume of cerebral infarction and the Evans blue permeability and the activity of MMP-9 of the ischemic brain tissue were significantly decreased (P <0.01) and the neurological deficits were significantly improved .Conclusion The atmospheric oxygen therapy during ischemia has a protective effect on the blood-brain barrier injury after cerebral ischemia-reperfusion in rats, and its mechanism may be through inhibiting the activity of MMP-9.