目的:消除复方甲硝唑含漱液的抑菌作用,检查其控制菌金黄色葡萄球菌。方法:采用薄膜过滤法,通过均匀设计从稀释剂、冲洗剂、冲洗次数3个因素,每个因素3个水平,以阳性菌回收率为指标优选复方甲硝唑含漱液的微生物限度检查法。结果:以0．5％聚山梨酯-80-0．9％无菌氯化钠溶液为稀释剂,0．5％蛋白胨-0．5％聚山梨酯-80溶液为冲洗剂,冲洗 3次,每次100 ml,取出滤膜做金黄色葡萄球菌的检查,阳性对照能正常检出。结论:该法能有效消除复方甲硝唑含漱液的抑菌作用,使测定结果准确、可靠。 Objective: To eliminate the antibacterial effect of compound metronidazole gargle and to check its control bacterium Staphylococcus aureus. Methods: By the method of membrane filtration, the three factors of diluent, irrigant, washing times and three factors were evenly designed to optimize the metronidazole gargle for microbial detection . Results: 0.5% polysorbate-80-0.9% sterile sodium chloride solution as a diluent, 0.5% peptone-0.5% polysorbate-80 solution as a rinse agent, rinsed three times , Each 100 ml, remove the filter to do staphylococcus aureus, positive control can be detected normally. Conclusion: The method can effectively eliminate the antibacterial effect of compound metronidazole gargle, so that the determination results are accurate and reliable.