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目的研究金丝桃苷、白麻苷和次野鸢尾黄素三种黄酮类化合物对不同肿瘤细胞和血管内皮细胞的细胞毒性。方法正常培养的乳腺癌细胞(MCF-7,MDA-MB-231)、肺腺癌细胞(A549)及血管内皮细胞(HUVECs)经不同浓度的金丝桃苷、白麻苷和次野鸢尾黄素(20,40,80,160μmol·L~(-1))分别处理24 h和48 h,倒置显微镜观察细胞形态,SRB法检测细胞存活率。结果金丝桃苷和白麻苷对三种肿瘤细胞均没有显著的细胞毒性,但抑制去除血清条件下HUVECs细胞凋亡;次野鸢尾黄素对乳腺癌MDA-MB-231细胞抑制较强,对乳腺癌MCF-7和肺癌A549细胞抑制较弱。结论金丝桃苷对内皮细胞具有保护功效,而次野鸢尾黄素是一种潜在的抗肿瘤黄酮类化合物。
Objective To study cytotoxicity of three flavonoids, including hyperoside, alanine and immature tectorigenin, on different tumor cells and vascular endothelial cells. Methods The normal cultured human breast cancer cells (MCF-7, MDA-MB-231), lung adenocarcinoma cells (A549) and vascular endothelial cells (HUVECs) were treated with different concentration of hyperoside, (20, 40, 80, 160μmol·L -1) for 24 h and 48 h, respectively. The morphological changes of the cells were observed under inverted microscope. The cell viability was detected by SRB. Results Hyperin and alanine had no cytotoxicity on the three kinds of tumor cells, but inhibited the apoptosis of HUVECs under serum-free condition. The effect of UTI on MDA-MB-231 breast cancer cells was stronger, The inhibition of breast cancer MCF-7 and lung cancer A549 cells is weak. Conclusions Hyperoside has a protective effect on endothelial cells, whereas iridovir is a potential anti-tumor flavonoid.