不同基因型大豆生长和结瘤对羟基磷灰石的吸收利用

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以来源于东北、西北和南方的15个不同基因型大豆品种作为供试材料,利用砂培培养方法,研究不同基因型大豆利用钙磷的差异。结果表明,钙磷对西北大豆生物量的促进作用最大,其次是南方品种,最后是东北品种。与东北品种比较,西北品种和南方品种生物量分别较东北品种增加32.9%和26.9%。垦农18、垦98-602和黑豆分别与相同来源的其它4个基因型大豆生物量达到5%的差异显著水平。通过比较和方差分析认为,钙磷对促进大豆根生物量增加比对地上部生物量增加的作用大。钙磷对南方和西北大豆根瘤形成的影响比东北的大。钙磷对南方大豆根瘤干重的促进作用最大,其次是东北大豆,最后是西北大豆。然而,方差分析表明,垦农18根瘤干重与东北的其它4个品种差异达到5%显著水平;西北的5个品种根瘤干重未达到差异显著水平;安选普定皂角豆的根瘤干重与其它4个南方品种差异达到5%的显著水平。相同来源的大豆地上部含磷量的平均值进行比较,东北品种地上部含磷量最高,达到2.23gkg-1,西北品种为2.14gkg-1,南方品种仅含有1.69gkg-1;根系含磷量是南方品种最高,达到2.03g kg-1,西北品种为1.66g kg-1,东北品种仅含有1.57g kg-1;根瘤含磷是西北品种最高,达到4.62g kg-1,东北品种为3.80g kg-1,南方品种为3.89g kg-1。不同来源大豆基因型根际pH差异较大,5个品种根际pH的平均值相比较,东北大豆根际pH最高,为6.17,其次是南方大豆为6.01,最低的是西北大豆,pH为5.81。方差分析表明,无论哪种来源的大豆,各品种根际pH都未达到差异显著水平。钙磷利用效率由高到低的顺序是南方、西北和东北品种,分析认为磷素利用效率并不能完全代表作物对钙磷的利用差异,综合作物对磷的吸收累积量将更有代表性。 Fifteen different soybean genotypes from Northeast, Northwest and South China were used as materials to study the difference of calcium and phosphorus utilization in different genotypes of soybean by sand culture method. The results showed that Ca and P had the greatest promotion effect on the biomass of northwest soybean, followed by the southern variety and finally the northeast variety. Compared with the northeast varieties, the biomass of the northwest varieties and the southern varieties increased by 32.9% and 26.9% respectively compared with the northeast varieties. Kenneth 18, Ken 98-602 and black soybean were significantly different from the other four genotypes with the same source at 5%. By comparison and analysis of variance, it is considered that the effects of Ca and P on the increase of root biomass of soybean are more than those of the aboveground biomass. The effect of Ca and P on the nodule formation of soybean in the south and northwest was larger than that of the northeast. Calcium and phosphorus had the greatest effect on the dry weight of southern soybean nodules, followed by northeast soybean and finally northwest soybean. However, analysis of variance showed that the difference of Keni 18 dry weight from other four varieties in northeast China reached 5%, and the dry weight of five varieties in northwest China did not reach the significant level. Difference with the other four southern varieties reached a significant level of 5%. Compared with the average phosphorus content of soybean in the same source, the content of phosphorus in aboveground part of Northeast China was the highest, reaching 2.23gkg-1, 2.14gkg-1 in northwestern China, and 1.69gkg-1 in south China. The highest level was 2.03g kg-1 in South China, 1.66g kg-1 in Northwest China, and 1.57g kg-1 in Northeast China. The highest phosphorus content in Northwest China was 4.62g kg-1 in nodules, 3.80g kg-1, and the southern variety is 3.89g kg-1. Compared with the average rhizosphere pH of five cultivars, rhizosphere pH of northeast soybean was the highest (6.17), followed by southern soybean (6.01), while the lowest was northwestern soybean with a pH of 5.81 . Analysis of variance showed that no matter what kind of source of soybean, the rhizosphere pH of all varieties did not reach the significant level of difference. The order of high and low utilization efficiency of calcium and phosphorus was south, northwest and northeast. The analysis indicated that the phosphorus use efficiency does not completely represent the difference of calcium and phosphorus utilization of crops, and the accumulation of phosphorus by integrated crop would be more representative.
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