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【目的】探讨大黄素抑制小鼠结肠癌发展与影响调节性T细胞(Tregs)迁徙的相关性。【方法】选用BALB/c小鼠随机分为阴性对照组、模型组和大黄素组(剂量为100 mg.kg-1.d-1),后2组采用右前肢腋窝皮下接种CT26细胞制备结肠癌模型;采用流式细胞仪检测Tregs在结肠癌小鼠外周血、引流淋巴结和肿瘤局部的相对比例,以及趋化因子受体CCR4在Tregs的表达量差异,并研究其与肿瘤大小的相关性。【结果】大黄素组的肿瘤质量显著低于模型组(P<0.05),抑瘤率达74%;大黄素组Tregs在肿瘤局部的比例及其CCR4+Tregs的比例显著低于荷瘤模型组(均P<0.01)。【结论】大黄素抑制小鼠结肠癌的发展与其影响Tregs的迁徙相关,其机制与大黄素降低了CCR4在Tregs上的表达有关。
【Objective】 To investigate the correlation between the inhibition of emodin on the development of colon cancer and the migration of regulatory T cells (Tregs) in mice. 【Methods】 BALB / c mice were randomly divided into negative control group, model group and emodin group (dose of 100 mg.kg-1.d-1), and the latter two groups were given CT26 cells subcutaneously in the right axilla Cancer. The relative proportion of Tregs in peripheral blood, draining lymph nodes and tumor in colon cancer mice was detected by flow cytometry, and the expression of chemokine receptor CCR4 in Tregs was also studied. The correlation between Tregs and tumor size . 【Results】 The tumor weight of emodin group was significantly lower than that of model group (P <0.05), and the tumor inhibition rate was 74%. The proportion of Tregs in tumor and the ratio of CCR4 + Tregs in emodin group was significantly lower than that in tumor-bearing model group (All P <0.01). 【Conclusion】 Emodin inhibits the development of colon cancer in mice and is related to its effect on the migration of Tregs. The possible mechanism is that emodin can reduce the expression of CCR4 in Tregs.