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目的:探讨血管紧张素转化酶(ACE)基因Alu I/D的多态性与心房颤动(Af)的关系。方法:研究对象均来自湖北地区汉族人群,120例Af患者(Af组),120例非Af者(对照组)。采用成组配比研究,取静脉血,提取基因组DNA,采用聚合酶链反应-限制性酶切片段长度多态性(PCR-RFLP)分析技术对2组人群ACE基因Alu I/D的多态性进行分析。结果:ACE DD基因型频率在Af组与对照组之间存在显著差异(32.5%∶18.3%,P=0.008),等位基因在2组间亦存在同样的趋势(D/I=52.9%∶37.5%,P=0.001)。在对混杂因素进行校正后,携带DD基因的人群患Af的危险性较高(OR=3.34,95%CI=1.58~7.04,P=0.002);携带ID基因型的人群患Af的危险性也升高,但差异无统计学意义(OR=1.95,95%CI=0.95~3.99,P=0.069);排除混杂因素后,左房内径与人群患Af的风险呈显著相关(OR=8.92,95%CI=3.72~21.40,P=0.000)。结论:在湖北地区汉族人群中,ACE基因Alu I/D的多态性与Af的发病呈明显相关性,可能是Af的遗传危险因素,左房内径的增加与Af的发病呈显著相关。
Objective: To investigate the relationship between angiotensin converting enzyme (ACE) gene Alu I / D polymorphism and atrial fibrillation (Af). Methods: All subjects were from Han nationality in Hubei Province, 120 Af patients (Af group) and 120 non-Af patients (control group). The ratio of Alu I / D polymorphism of ACE gene in two groups was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Sex analysis. Results: The frequency of ACE DD genotypes was significantly different between Af group and control group (32.5%: 18.3%, P = 0.008). There was also the same tendency of allele between two groups (D / I = 52.9% 37.5%, P = 0.001). After adjusting for confounding factors, the risk of Af was higher in those with DD gene (OR = 3.34, 95% CI = 1.58-7.04, P = 0.002); those with ID genotype also had a greater risk of Af (OR = 1.95,95% CI = 0.95-3.99, P = 0.069). After excluding confounding factors, there was a significant correlation between the left atrial diameter and the risk of Af in the population (OR = 8.92, 95 % CI = 3.72 ~ 21.40, P = 0.000). CONCLUSION: Apoptosis of ACE gene Alu I / D polymorphism in Han population of Hubei Province is significantly associated with the onset of Af, which may be the genetic risk factor for Af. The increase of left atrial diameter is significantly associated with the onset of Af.