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目的观察抑制转化生长因子(TGF)β1表达对大肠癌细胞生长周期和侵袭力的影响。方法用小片段TGβ1的双链RNA(siRNA)转染大肠癌HCT116细胞,半定量RT-PCR法检测 TGFβ1受抑程度,流式细胞仪观察转染后细胞周期变化,软琼脂克隆实验检测肿瘤细胞生长及成瘤性的变化。结果 TGFβ1的小片段RNA转染显著抑制了肿瘤细胞TGFβ1的表达,使细胞HCT116的S 期细胞较对照组明显增高59．0％-82．5％,进入G2期细胞减少;转染组细胞克隆形成率较对照组显著下降。结论 RNA干扰技术可快捷方便地阻断TGFβ1表达,使大肠癌细胞S期阻滞并抑制肿瘤细胞生长、降低其成瘤性。
Objective To investigate the effect of inhibiting the expression of transforming growth factor β1 (TGF-β1) on the growth and invasiveness of colorectal cancer cells. Methods Human colon cancer HCT116 cells were transfected with a small fragment of TGβ1 double stranded RNA (siRNA). The degree of inhibition of TGFβ1 was detected by semi-quantitative RT-PCR. The cell cycle was observed by flow cytometry. The soft agar assay Growth and tumorigenic changes. Results Small RNA transfected with TGFβ1 significantly inhibited the expression of TGFβ1 in tumor cells and increased the number of S phase cells in HCT116 cells by 59.0% -82.5% compared with that in control cells. The number of cells entering G2 phase decreased. The transfected cells The formation rate was significantly lower than the control group. Conclusion RNAi can block the expression of TGFβ1 rapidly and conveniently, block the S phase of colorectal cancer cells, inhibit the growth of tumor cells and reduce the tumorigenicity.