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为了寻求高效的血管紧张素转换酶(ACE)抑制肽制备方法,在实验室条件下利用碱性蛋白酶水解豆粕生产ACE抑制肽,并采用单因素和多因素实验相结合的方法对酶解条件进行优化.结果发现,适当延长碱性蛋白酶的水解时间和提高p H,可大幅度提高ACE抑制肽的抑制活性.单因素和多因素实验综合结果显示,碱性蛋白酶水解豆粕产生ACE抑制肽的适宜水解条件为:p H为9.0,水解温度为50℃,水解时间为4 h,蛋白酶质量分数(酶质量/底物质量)为2.0%,底物质量浓度为40 g/L,在此条件下制备的ACE抑制肽的抑制率可达到97.97%.
In order to find an efficient preparation method of angiotensin converting enzyme (ACE) inhibitory peptide, ACE inhibitory peptides were produced by using alkaline protease to hydrolyze soybean meal under laboratory conditions, and the conditions of enzymolysis were carried out by a combination of single factor and multi-factor experiments The results showed that prolonging the hydrolysis time of alkaline protease and increasing p H could greatly improve the inhibitory activity of ACE inhibitory peptides.The comprehensive results of single factor and multifactorial experiments showed that the hydrolysis of soybean protein by alkaline protease was suitable for ACE inhibitory peptides The hydrolysis conditions were as follows: p H 9.0, hydrolysis temperature 50 ℃, hydrolysis time 4 h, protease mass fraction (enzyme mass / substrate mass) 2.0%, substrate concentration 40 g / L, under these conditions The inhibition rate of the prepared ACE inhibitory peptide can reach 97.97%.