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目的:运用双能X线吸收法(DXA)和micro-CT技术评价波尔定对Ⅱ型胶原诱导性关节炎(CIA)大鼠腰椎及股骨近端骨质骨微结构的影响。方法:选用雄性SD大鼠,8只作为空白对照组,其余大鼠制作CIA模型,再随机分为CIA模型组、甲氨蝶呤(MTX)组、波尔定低剂量组和波尔定高剂量组。免疫第15d时开始灌胃给药,连续给药28d后,取大鼠腰椎行骨密度检测,右侧股骨行micro-CT检测并进行三维重建。结果:与CIA模型组比较,波尔定高剂量组大鼠腰椎骨密度显著升高(P<0.01)。股骨近端骨微结构结果:与CIA模型组相比,波尔定高剂量组骨体积分数、骨小梁数量、骨小梁厚度均显著升高(P<0.05,P<0.01),而骨小梁分离度、骨表面积/体积、骨小梁模式因子显著下降(P<0.05,P<0.01)。结论:高剂量波尔定可明显改善CIA大鼠腰椎和股骨近端骨质骨微结构状态,提示其对类风湿关节炎继发的全身性骨质疏松可能具有潜在的预防作用。
OBJECTIVE: To evaluate the effects of diazepam on the microstructure of the lumbar vertebra and proximal femur of type Ⅱ collagen-induced arthritis (CIA) rats by dual-energy X-ray absorptiometry (DXA) and micro-CT. Methods: Eight male Sprague-Dawley rats were used as blank control group. The other rats were divided into CIA model group, methotrexate (MTX) group, low dose oxaliplatin group and high dose of oxaliplatin Dose group. Immunization 15d began gavage, continuous administration 28d, take the lumbar spine bone density test, the right femur micro-CT detection and three-dimensional reconstruction. Results: Compared with the CIA model group, the lumbar BMD of high dose oxaliplatin group was significantly increased (P <0.01). Compared with CIA model group, the bone volume fraction, trabecular number and trabecular thickness were significantly increased in high-dose oxaliplatin group (P <0.05, P <0.01) Trabecular separation, bone surface area / volume and trabecular pattern factor were significantly decreased (P <0.05, P <0.01). CONCLUSION: High dose of porgine can significantly improve the microstructure of lumbar spine and femoral proximal femur in CIA rats, suggesting that it may have a potential preventive effect on systemic osteoporosis secondary to rheumatoid arthritis.