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从茶多酚生产的下脚料中,通过有机溶剂洗涤和凝胶色谱分离,得到一种水溶性多糖。经高效液相色谱分析,为单一茶多糖,其相对分子量为(10～10.5)×105;气相色谱分析表明,糖基由葡萄糖、半乳糖、阿拉伯糖、甘露糖和鼠李糖组成,其摩尔比为6.97∶5.34∶8.11∶2.76∶1.14;比色法测其总糖含量、蛋白含量、糖醛酸含量分别为90%、8.52%、27.65%。红外光谱和核磁共振分析表明,该多糖是由单糖通过β-糖苷键连接的,多糖链上络有糖醛酸、氨基或蛋白基团,同时含有α和β异头碳;x-衍射分析显示,随多糖纯度的提高,茶多糖更易结晶,且有不同的晶体聚合物出现;DSC的分析表明,随茶多糖纯度的提高,热焓增加,峰温向高温漂移。
From the waste of tea polyphenol production, a water-soluble polysaccharide is obtained by organic solvent washing and gel chromatography. The relative molecular weight of single tea polysaccharide was (10 ~ 10.5) × 105 by high performance liquid chromatography. The gas chromatography analysis showed that the sugar group consisted of glucose, galactose, arabinose, mannose and rhamnose, Ratio of 6.97:5.34:8.11:2.76:1.14. The total sugar content, protein content and uronic acid content were 90%, 8.52% and 27.65% respectively by colorimetry. IR and 1H NMR showed that the polysaccharides were linked by monosaccharides through β-glycosidic bonds. The polysaccharides chains had uronic acid, amino or protein groups and both α and β anomeric carbons. X-ray diffraction The results showed that with the increase of the purity of polysaccharides, tea polysaccharides were easier to crystallize and different crystalline polymers appeared. The analysis of DSC showed that the enthalpy increased with the increase of purity of tea polysaccharides, and the peak temperature shifted to high temperature.