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目的:观察脱矿牙本质基质(demineralized dentin matrix,DDM)与大鼠骨髓间充质干细胞(rat bone marrow mesenehymal stem ceils,rBMSCs)的生物相容性及其作为支架材料对BMSCs的牙向诱导作用。方法:分离培养BMSCs,分别用四唑盐比色法(MTS)、茜素红染色法、碱性磷酸酶(alkaline phosphatase,ALP)和逆转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)方法观察DDM生物材料对BMSCs增殖活性和牙向分化能力的影响。结果:DDM能显著促进体外培养的BMSCs的增殖、诱导细胞的矿化和提高细胞ALP活性;同时还能诱导BMSCs在mRNA水平表达牙本质涎磷蛋白(dentin sialophosphoprotein,DSPP)和牙本质基质蛋白(dentin matrix protein1,DMP-1)。结论:体外培养条件下,DDM与BMSCs有良好的生物相容性,能够诱导BMSCs牙向分化。
OBJECTIVE: To observe the biocompatibility of demineralized dentin matrix (DDM) and rat bone marrow mesenchymal stem cells (rBMSCs) and their dental effects on BMSCs as scaffolds . Methods: BMSCs were isolated and cultured. The expression of BMSCs was detected by MTT assay, alizarin red staining, alkaline phosphatase (ALP) and reverse transcription-polymerase chain reaction RT-PCR) method was used to observe the effects of DDM biomaterials on proliferation activity and dental differentiation ability of BMSCs. RESULTS: DDM significantly promoted the proliferation of BMSCs cultured in vitro, induced the mineralization of cells and increased the activity of ALP. At the same time, DDM also induced the expression of dentin sialophosphoprotein (DSPP) and dentin matrix protein dentin matrix protein1, DMP-1). Conclusion: Under the conditions of in vitro culture, DDM and BMSCs have good biocompatibility and can induce the differentiation of BMSCs.