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目的观察研究FOXP3蛋白在颈段食管癌细胞中的表达及肿瘤间质浸润的FOXP3+淋巴细胞数量,探讨其临床意义。方法通过免疫组织化学方法、免疫组化双染技术,对42例颈段食管癌的肿瘤组织和30例的癌旁组织的石蜡切片进行分析,观察FOXP3蛋白的表达FOXP3+淋巴细胞的浸润。结果 FOXP3蛋白在颈段食管癌实质细胞和癌旁实质细胞表达的阳性率分别为42.9%(18/42)和6.67%(2/30),两者差异有统计学意义(χ2=10.324,P<0.01)。颈段食管癌和癌旁组织间质浸润的FOXP3+淋巴细胞阳性率分别为38.1%(16/42)和13.33%(4/30),两者差异有统计学意义(χ2=15.376,P<0.01)。颈段食管癌实质细胞中,周围淋巴结转移组FOXP3蛋白表达显著性高于无淋巴结转移组(P<0.05);同时临床分期Ⅲ、Ⅳ期标本中FOXP3蛋白表达显著性高于Ⅰ、Ⅱ期标本(P<0.05)。在淋巴结转移组中,肿瘤间质中浸润FOXP3+淋巴细胞数量明显多于无淋巴结转移组,表达有显著性差异(P<0.05)。结论颈段食管癌实质细胞FOXP3表达异常和FOXP3+淋巴细胞浸润可能与颈段食管癌的转移关系密切,能够促进肿瘤免疫逃逸的发生;FOXP3可作为评估颈段食管癌术后转移的一种潜在标记物。
Objective To investigate the expression of FOXP3 protein in cervical esophageal cancer cells and the number of FOXP3 + lymphocytes infiltrating into the tumor interstitium and to explore its clinical significance. Methods Immunohistochemistry and immunohistochemical double staining were used to analyze the expression of FOXP3 in FOXP3 + lymphocytes in 42 cases of cervical esophageal cancer and 30 cases of paraffin-embedded paraffin sections. Results The positive rates of FOXP3 protein expression in cervical esophageal cancer and para-cancerous parenchyma cells were 42.9% (18/42) and 6.67% (2/30), respectively, with significant difference (χ2 = 10.324, P <0.01). The positive rates of interstitial infiltration of FOXP3 + lymphocytes in cervical esophageal cancer and paracancerous tissues were 38.1% (16/42) and 13.33% (4/30), respectively, with significant difference (χ2 = 15.376, P <0.01) ). The expression of FOXP3 protein in the cervical esophageal cancer cells was significantly higher than that in the non-lymph node metastasis group (P <0.05). Meanwhile, FOXP3 protein expression in stage Ⅲ and Ⅳ clinical specimens was significantly higher than that in stage Ⅰ and Ⅱ (P <0.05). In lymph node metastasis group, the number of infiltrating FOXP3 + lymphocytes in tumor interstitium was significantly higher than that in non-lymph node metastasis group (P <0.05). Conclusions The abnormal expression of FOXP3 and FOXP3 + lymphocyte infiltration in cervical esophageal cancer cells may be closely related to the metastasis of cervical esophageal cancer and promote the escape of tumor immunity. FOXP3 may be used as a potential marker to evaluate the metastasis of cervical esophageal cancer Things.