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目的:探讨广泛耐药鲍曼不动杆菌对氨基糖苷类耐药机制。方法:应用西门子MicroScan微生物鉴定及药敏系统对2012年1月到2014年1月临床分离得到的4500例鲍曼不动杆菌进行细菌培养鉴定及药敏分析,聚合酶链反应(PCR)方法分析8种氨基糖苷类修饰酶基因、5种l6SrRNA甲基化酶基因和外排泵adeABC基因系统。结果:4500株鲍曼不动杆菌均检出adeB外排基因、armA和aac(3)-I、aac(6’)-I、ant(3″)-I基因,其余5种氨基糖苷类修饰酶基因和4种16SrRNA甲基化酶基因检出率很小。结论:鲍曼不动杆菌对氨基糖苷类抗生素的耐药机制与细菌产生的adeB外排基因、armA这一种16SRNA甲基化酶基因和aac(3)-I、aac(6’)-I、ant(3″)-I这3种氨基糖苷类修饰酶基因有关。
Objective: To investigate the mechanism of drug resistance to aminoglycosides by Acinetobacter baumannii. Methods: The clinical culture of 4500 Acinetobacter baumannii isolates from January 2012 to January 2014 were identified and drug-susceptible by using the MicroScan Microbial Identification and Drug-Sensitive System of Siemens. The polymerase chain reaction (PCR) method Eight aminoglycoside modifying enzyme genes, five l6SrRNA methylase genes and an efflux pump adeABC gene system. Results: All the 4 strains of A. baumannii were detected adeB efflux gene, armA and aac (3) - I, aac (6 ’) - I and ant (3 “) - I genes and the remaining 5 aminoglycoside The detection rate of gene and four kinds of 16SrRNA methylase genes was very small.Conclusion: The mechanism of resistance to aminoglycoside antibiotics in A. baumannii is related to the adeB efflux gene produced by bacteria, armA, a 16S RNA methylation The enzyme gene is associated with three aminoglycoside modifying enzyme genes, aac (3) -I, aac (6 ’) - I and ant (3 ”) - I.