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本研究利用反转录聚合酶链式反应(RT-PCR)观察了将氯胺酮给予生后早期大鼠后,海马组织中NMDA受体亚型mR-NA的表达变化。实验用生后7d的SD大鼠40只,随机分为2大组:给药后即刻组(PND7组)和给药后3周组(PND28组)。PND7组为腹腔注射不同剂量氯胺酮后24h内处死,PND28组用相同给药方法给药并在相同环境中饲养3周(即至生后28d)后处死。RT-PCR结果显示在PND7组,腹腔注射氯胺酮引起海马组织内NR2A,NR2B和NR2C亚型mRNA的表达上调,NR1受体亚型mRNA的表达无明显变化。在PND28组,氯胺酮注射引起NR1和NR2A亚型mRNA的表达水平增高,而NR2B和NR2C亚型mRNA的表达没有变化。氯胺酮的给药量和给药方式对上述受体亚型的表达变化没有明显影响。各受体亚型在不同发育阶段的海马组织中的表达水平也有一定的差异。本研究的结果提示氯胺酮对发育阶段大鼠脑内NMDA受体的表达具有上调作用,从而对大鼠神经系统的发育可能产生影响。
In this study, the expression of NMDA receptor subtypes mR-NA in hippocampus after ketamine was administered to early postnatal rats was investigated by reverse transcription-polymerase chain reaction (RT-PCR). Forty SD rats were randomly divided into 2 groups: PND7 group and PND28 group after administration. PND7 group were intraperitoneally injected with different doses of ketamine after 24 hours of death, PND28 group administered by the same method of administration and feeding in the same environment for 3 weeks (ie, 28 days after birth) were sacrificed. RT-PCR results showed that in the PND7 group, the expression of NR2A, NR2B and NR2C subtypes mRNA was upregulated in hippocampus of rats in PND7 group, while the expression of NR1 receptor subtype mRNA did not change significantly. In PND28 group, ketamine induced increased expression of NR1 and NR2A subtypes mRNA, while NR2B and NR2C subtypes mRNA expression did not change. The amount of ketamine administered and the mode of administration did not significantly affect the expression of the above receptor subtypes. The expression of each receptor subtype in different developmental stages of the hippocampus also have some differences. The results of this study suggest that ketamine may up-regulate the expression of NMDA receptors in the developing rat brain, which may have an impact on the development of the nervous system in rats.