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报道了灵芝对Balb/c小鼠产生抗SRBC抗体能力的调节及产生γ-干扰素的影响。实验组小鼠分成4组,分别经胃灌注不同灵芝制剂:1号、2号、3号及4号制剂,每天一次,连续9天,对照组小鼠则以等量蒸馏水代替。在实验第7天用SRBC免疫小鼠,于免疫后第4天进行溶血空斑试验和血清抗SRlBC抗体凝集效价测定,并取小鼠脾细胞加PHA刺激、诱导,于37℃、5%CO_2培养72小时,测定培养液中γ-干扰素活性。结果表明,1号、2号制剂能明显提高实验组小鼠的溶血空斑数目及血清抗体凝集效价,与对照组相比,P<0.05,2号、4号制剂则能明显提高实验组小鼠产生的γ-干扰素活性,与对照组相比,P<0.05。提示灵芝能增强小鼠的免疫功能─—B细胞产生特异性抗体的能力及T细胞产生(经PHA诱导)的γ-干扰素的能力。
The effect of Ganoderma lucidum on the production of anti-SRBC antibodies in Balb/c mice and the effect of gamma-interferon production were reported. The mice in the experimental group were divided into 4 groups, and different Ganoderma preparations were intragastrically perfused: Formula 1, No. 2, No. 3, and No. 4 preparations, once a day for 9 days, and mice in the control group were replaced with equal amounts of distilled water. On the 7th day of the experiment, mice were immunized with SRBC. Hemolysis plaque assay and serum anti-SRlBC antibody agglutination titer assay were performed on the 4th day after immunization. Murine spleen cells were stimulated and induced with PHA at 37[deg.] C. and 5%. CO2 was cultured for 72 hours and the gamma-interferon activity in the culture fluid was measured. The results showed that No. 1 and No. 2 preparations can significantly increase the number of hemolytic plaques and serum antibody agglutination titer in the experimental group. Compared with the control group, P<0.05, No. 2 and No. 4 preparations can be significantly improved. The activity of γ-interferon produced by mice in the experimental group was P<0.05 compared with the control group. It suggests that Ganoderma lucidum can enhance the immune function of mice: the ability of B cells to produce specific antibodies and the ability of T cells to produce (via PHA-induced) gamma-interferon.