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目的探讨锰超氧化物歧化酶9 Ala/Val(MnSOD9 Ala/Val)基因多态性与冠心病、总超氧化物歧化酶(T-SOD)和锰超氧化物歧化酶(MnSOD)活性以及丙二醛(MDA)浓度的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测262例冠心病患者和100例对照组的MnSOD9 Ala/Val基因多态性的基因型,采用比色法测定血浆T-SOD、MnSOD活性以及MDA浓度。结果与对照组比较,冠心病患者的血浆T-SOD和MnSOD活性[(23.61±16.51)U/ml与(44.01±22.68)U/ml,P<0.001和(21.56±13.11)U/ml与(28.79±8.65)U/ml,P<0.001]明显降低,MDA浓度显著增高[(2.47±0.73),(2.15±0.55)nmol/ml,P<0.01];冠心病患者的MnSOD 9 AA基因型和A等位基因频率较对照组明显增多(64.2%与43.0%,P=0.001和80.0%与67.0%,P=0.014);MnSODAA基因型的MnSOD活牲较VV基因型明显降低[(22.87±13.47)与(32.04±9.19)U/ml,P<0.01)];MnSOD与MDA负相关(r=-0.15,P<0.01)。结论冠心病患者的血浆T-SOD和MnSOD活性明显降低,MDA浓度显著增高;MnSOD 9 Ala/Val基因多态性AA基因型的血浆MnSOD活性降低。
Objective To investigate the relationship between Mn (superscript +) Ala 9 Val / Val (MnSOD9 Ala / Val) gene polymorphism and coronary heart disease, total superoxide dismutase (T-SOD) and manganese superoxide dismutase (MnSOD) Dialdehyde (MDA) concentration of the relationship. Methods The genotypes of MnSOD9 Ala / Val gene polymorphism in 262 CHD patients and 100 control subjects were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The plasma levels of plasma T-SOD, MnSOD activity and MDA concentration. Results Compared with the control group, the plasma levels of T-SOD and MnSOD in patients with coronary heart disease (23.61 ± 16.51 U / ml and 44.01 ± 22.68 U / ml, P <0.001 and (21.56 ± 13.11) U / (2.47 ± 0.73) and (2.15 ± 0.55) nmol / ml respectively, P <0.01]. The genotypes of MnSOD 9 AA in patients with coronary heart disease were significantly lower than those in patients with coronary heart disease (28.79 ± 8.65 U / ml, P 0.001) The frequency of allele A was significantly higher than that of the control (64.2% vs 43.0%, P = 0.001 and 80.0% vs 67.0%, P = 0.014). The MnSOD activity of MnSODAA genotype was significantly lower than that of VV genotype ([22.87 ± 13.47 ) And (32.04 ± 9.19) U / ml, P <0.01). MnSOD was negatively correlated with MDA (r = -0.15, P <0.01). Conclusions Plasma T-SOD and MnSOD activities in patients with coronary heart disease were significantly lower and MDA levels were significantly higher. The activity of MnSOD in MnSOD 9 Ala / Val polymorphism AA genotype was decreased.