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目的:应用寡聚核苷酸基因表达谱芯片研究鼻息肉(NP)基因表达谱的变化。方法:应用包含14500条人类全长基因的寡聚核苷酸芯片HG-U133A2.0检测6例NP组织,6例正常鼻黏膜组织的基因表达谱,分析差异表达基因,并选择其中有差异表达的部分基因进行realtime RT-PCR验证。结果:基因芯片筛选出大量差异表达的基因,其中包括炎性反应、免疫应答、免疫调控及信号传导的相关基因及其受体,如IL-8、RGS1、GRK4、CCL20、子宫球蛋白(uteroglobin)等。实时荧光定量PCR测定差异表达基因IL-8、RGS1与基因芯片结果一致。结论:NP基因表达谱存在差异,RGS1可能通过影响细胞信号传导通路发挥作用,IL-8可能通过促使炎性细胞释放多种炎性递质参与鼻息肉发病。
Objective: To study the changes of nasal polyps (NP) gene expression profile by oligonucleotide microarray. Methods: The gene expression profiles of 6 NP tissues and 6 normal nasal mucosa tissues were detected by using HG-U133A2.0, an oligonucleotide microarray containing 14500 human full-length genes. The differentially expressed genes were analyzed and differentially expressed Part of the gene for realtime RT-PCR validation. Results: Gene microarray screened a large number of differentially expressed genes, including inflammatory response, immune response, immune regulation and signal transduction related genes and their receptors, such as IL-8, RGS1, GRK4, CCL20, uteroglobin )Wait. Real-time fluorescence quantitative PCR assay of differentially expressed genes IL-8, RGS1 and gene chip results. CONCLUSIONS: There are differences in NP gene expression profiles. RGS1 may play a role in cell signal transduction pathway. IL-8 may participate in the pathogenesis of nasal polyps through promoting the release of inflammatory mediators by inflammatory cells.