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目的探讨免疫球蛋白对戊四氮致惊大鼠海马神经元死亡及IL-1β和IL-1 ra表达的影响。方法在W istar大鼠腹腔内注射戊四氮(pentylenetetrazol,PTZ)诱发大鼠惊厥发作,制作癫模型。将45只大鼠随机分为3组,正常对照组,PTZ静脉注射免疫球蛋白组(PTZ-IVIG),PTZ-生理盐水组(PTZ-NS)。经HE和TUNEL染色,在光镜下,观察神经细胞死亡,采用免疫组织化学方法检测神经细胞内IL-1β和IL-1 ra表达。结果海马CA1区IL-1β/IL-1 ra比值PTZ-IVIG组(0.5±0.1)较PTZ-NS组(1.9±0.5)低,两者比较差异有统计学意义(t=12.9,P<0.05)。PTZ-IVIG组海马CA1区凋亡细胞数较PTZ-NS组少,阳性细胞数前者为(16.4±3.3)个/1000μm2,后者为(41.7±3.5)个/1000μm2,两者差异有统计学意义(t=27.1,P<0.05)。PTZ-IVIG组海马CA1区坏死细胞数少,为(19.0±2.6)个/1000μm2,PTZ-NS组为(42.3±4.9)个/1000μm2,两者差异有统计学意义(t=20.9,P<0.05)。结论免疫球蛋白具有抑制惊厥所致的神经元死亡的作用,其作用可能机制为调节神经细胞内白介素1网络系统。
Objective To investigate the effects of immunoglobulin on the neuronal death of hippocampus and the expression of IL-1β and IL-1 ra in rats induced by pentylenetetrazole. Methods Epileptic rats were induced by injection of pentylenetetrazol (PTZ) intraperitoneally in Wistar rats. Forty five rats were randomly divided into three groups: normal control group, PTZ-IVIG group and PTZ-NS group. The cells were stained with HE and TUNEL, and the death of neurons was observed under light microscope. The expression of IL-1β and IL-1 ra in nerve cells were detected by immunohistochemistry. Results The IL-1β / IL-1 ra ratio in hippocampal CA1 region was lower in PTZ-IVIG group than in PTZ-NS group (1.9 ± 0.5) (P <0.05) ). Compared with PTZ-NS group, the number of apoptotic cells in hippocampal CA1 area of PTZ-IVIG group was (16.4 ± 3.3) / 1000μm2 and (41.7 ± 3.5) / 1000μm2 respectively, the difference was statistically significant Significance (t = 27.1, P <0.05). The number of necrotic cells in hippocampus CA1 of PTZ-IVIG group was (19.0 ± 2.6) / 1000μm2 and (42.3 ± 4.9) / 1000μm2 respectively in PTZ-NSIG group (t = 20.9, P < 0.05). Conclusion Immunoglobulin can inhibit neuronal death induced by convulsion and its possible mechanism is to regulate interleukin-1 network in neurons.