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目的:建立高效液相色谱波长切换法对牡丹皮中6个成分(没食子酸、氧化芍药苷、芍药苷、1,2,3,4,6-五没食子酰葡萄糖、丹皮酚、苯甲酰芍药苷)进行分析。方法:采用Phenomsil ODS(4.6 mm×250 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸水(B)为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长为267 nm(0～20 min没食子酸)、258 nm(20～30 min氧化芍药苷)、230 nm(30～50 min,芍药苷)、274 nm(50～80 min 1,2,3,4,6-五没食子酰葡萄糖、丹皮酚)、230 nm(80～90 min苯甲酰芍药苷),柱温30℃。结果:牡丹皮中6个成分没食子酸、氧化芍药苷、芍药苷、1,2,3,4,6-五没食子酰葡萄糖、丹皮酚、苯甲酰芍药苷进样量分别在0.109 4～1.094μg(r=0.999 5),0.034 86～0.348 6μg(r=0.999 5),0.212 4～2.124μg(r=0.999 1),0.214 5～2.145μg(r=0.999 6),0.323 5～3.235μg(r=0.999 4),0.075 84～0.758 4μg(r=0.999 3)与峰面积呈良好线性关系;平均回收率(n=6)分别为98.7%,98.8%,99.7%,98.3%,98.9%,99.2%。结论:该方法准确可靠、重复性好,可用于牡丹皮的质量控制。
OBJECTIVE: To establish a high performance liquid chromatography (HPLC) wavelength-switching method for the determination of 6 components in moutan bark (gallic acid, paeoniflorin, paeoniflorin, 1,2,3,4,6-decalin, paeonol, Paeoniflorin) for analysis. Methods: The mobile phase was eluted with a gradient of acetonitrile (A) -0.1% phosphoric acid (B) using a mobile phase of Phenomsil ODS (4.6 mm × 250 mm, 5 μm) with a flow rate of 1.0 mL · min- (0-20 min gallic acid), 258 nm (20-30 min oxidized paeoniflorin), 230 nm (30-50 min paeoniflorin), 274 nm (50-80 min 1,2,3,4,6 - gallate, paeonol) at 230 nm (80-90 min benzoyl paeoniflorin) at 30 ° C. Results: The contents of 6 components of paeoniflorin, such as gallic acid, paeoniflorin, paeoniflorin, 1,2,3,4,6-decalciferol, paeonol and paeoniflorin were 0.109 4 ~ (R = 0.999 5), 0.2344 ~ 2.145μg (r = 0.999 6), 0.323 5 ~ 3.235μg (r = 0.999 5), 0.2344 ~ 2.144μg (r = 0.999 4), 0.075 84 ~ 0.758 4μg (r = 0.999 3) showed a good linear relationship with the peak area. The average recoveries were 98.7%, 98.8%, 99.7%, 98.3% and 98.9% , 99.2%. Conclusion: The method is accurate and reliable with good repeatability and can be used for the quality control of Cortex Moutan.