Genetic analysis of fertility restoring genes for AL-type male sterility in wheat

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In order to screen molecular markers linked to fertility restoring genes and further improve the breeding efficiency of restorer lines,in this study,wheat varieties 18A,18B and 99AR144-1 were used as experimental materials to establish F2 fertility-segregating population. Plant quantitative trait“major gene + polygene mixed model”separation analysis method and simple sequence repeat(SSR) molecular markers were adopted for genetic analysis of four generations,including the parents(P1 and P2),and hybrid(Fl and F2) populations. The results show that AL-type fertility restoring gene is controlled by two pairs of additive-dominant-epistatic genes and additive-dominant polygene;two primers linked to fertility restoring genes were selected by SSR molecular markers, including Xgwm95 on chromosome 2A and Barc61 on chromosome 1B,with the linkage distance of 15.0 cM and 18.0 cM,respectively. Based on verification,these two markers are reliable for distinguishing AL-type wheat sterile lines and restorer lines. In order to screen molecular markers linked to fertility restoring genes and further improve the breeding efficiency of restorer lines, in this study, wheat varieties 18A, 18B and 99 AR144-1 were used as experimental materials to establish F2 fertility-segregating population. Plant quantitative trait “major gene + polygene mixed model ” separation analysis method and simple sequence repeat (SSR) molecular markers were adopted for genetic analysis of four generations, including the parents (P1 and P2), and hybrid (Fl and F2) populations. results show that AL-type fertility restoring gene is controlled by two pairs of additive-dominant-epistatic genes and additive-dominant polygene; two primers linked to fertility restoring genes were selected by SSR molecular markers, including Xgwm95 on chromosome 2A and Barc61 on chromosome 1B, with the linkage distance of 15.0 cM and 18.0 cM, respectively. Based on verification, these two markers are reliable for distinguishing AL-type wheat sterile lines and restorer lines.
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