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目的 探讨河南食管癌高发区居民食管癌发生发展的基因组变化特征。方法 应用比较基因组杂交技术分析52例原发性食管癌患者染色体基因组变化,按临床分期、有无淋巴结及远处转移进行分组比较。结果 在食管癌中3q、8q、5p、1q、6q、18p、20q的染色体基因组扩增和3p、1p、9q、19p、4p、8p染色体基因组丢失频繁( >20% )。3q、5p、1q、11q13 14的染色体基因组扩增和4pq、13q染色体基因组丢失与食管癌病理分期相关(P<0 .05)。8q扩增和4p丢失与淋巴结转移相关(P<0. 05)。2p扩增和4pq、l1q14 qter的丢失与远处器官转移相关(P<0 .05)。结论 染色体3q、8q、5p、1q、6q、18p和20q部位可能存在与食管癌变密切相关的癌基因, 3p、1p、9q、19p、4p和8p可能存在与食管癌变密切相关的抑癌基因; 3q、5p、1q、11q13 14扩增和4pq、13q丢失与食管癌的发展相关,而8q、2p扩增和4pq、11q14 qter的丢失是食管癌发展的晚期事件与食管癌转移相关,不同的基因参与了淋巴结转移和远处器官转移。
Objective To investigate the genomic changes of esophageal cancer in Henan esophageal cancer high incidence areas. Methods Genomic hybridization was used to analyze the chromosomal genomic changes in 52 patients with primary esophageal cancer, and compared by clinical stage, with or without lymph node and distant metastasis. Results Chromosome genomic amplification of 3q, 8q, 5p, 1q, 6q, 18p, 20q and frequent (> 20%) loss of 3p, 1p, 9q, 19p, 4p, 8p chromosomal genomes in esophageal cancer. The chromosome genomic amplification of 3q, 5p, 1q and 11q1314 and the loss of 4pq and 13q chromosomes were associated with the pathological stage of esophageal cancer (P <0.05). 8q amplification and 4p loss associated with lymph node metastasis (P <0.05). 2p amplification and 4pq, l1q14 qter loss associated with distant organ metastases (P <0 .05). Conclusion There may be oncogenes closely related to esophageal carcinogenesis at chromosomes 3q, 8q, 5p, 1q, 6q, 18p and 20q. 3p, 1p, 9q, 19p, 4p and 8p may have tumor suppressor genes closely related to esophageal cancer. 3q, 5p, 1q, 11q1314 amplification and 4pq, 13q loss associated with the development of esophageal cancer, and 8q, 2p amplification and 4pq, 11q14 qter loss of esophageal cancer is the development of advanced events associated with esophageal cancer metastasis, different Genes involved in lymph node metastasis and distant organ metastases.