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目的:研究舍格伦综合征(SS)患者血清中骨桥蛋白(OPN)等细胞因子的含量及相互关系。方法:经病理学检查确诊的SS患者40例,其中原发性15型,继发性25例。酶联免疫吸附试验(ELISA)检测患者血清中骨桥蛋白,肿瘤坏死因子-α(TNF-α),转化生长因子-β1(TGF-β1)的含量。以20名健康志愿者作为正常对照。结果:与正常对照组相比,OPN和TNF-α水平在SS患者显著增加(P<0.05);继发性SS的TGF-β1血清水平显著高于原发性SS,但OPN和TNF-α的血清水平在原发性SS与继发性SS中没有显著差异(P>0.05)。结论:骨桥蛋白在原发性SS和继发性SS患者的血清中表达均高于正常对照组,但继发性SS较原发性SS表达更为显著。说明其表达与疾病的严重程度有关。继发性SS的TGF-β1血清水平显著高于原发性SS,说明继发性SS疾病的病理发生发展更为复杂。
Objective: To study the serum levels of osteopontin (OPN) and other cytokines in patients with Sjogren’s syndrome (SS). Methods: 40 cases of SS patients diagnosed by pathology, of which 15 were primary and 25 were secondary. Serum levels of osteopontin, tumor necrosis factor-α (TNF-α), and transforming growth factor-β1 (TGF-β1) were detected by enzyme linked immunosorbent assay (ELISA). Twenty healthy volunteers were used as normal controls. Results: Compared with normal control group, the levels of OPN and TNF-α were significantly increased in SS patients (P <0.05); the serum levels of TGF-β1 in secondary SS were significantly higher than those in primary SS, but OPN and TNF-α Serum levels did not differ significantly between primary SS and secondary SS (P> 0.05). CONCLUSION: The expression of osteopontin in the serum of patients with primary SS and secondary SS is higher than that of the normal control group, but the secondary SS is more significant than the primary SS. Indicating that the expression of the disease severity. The level of serum TGF-β1 in secondary SS was significantly higher than that of primary SS, which indicated that the pathological development of secondary SS was more complicated.